Enzyme Kinetics Flashcards
What are the two types of irreversible inhibitions
Covalent Bonding
Suicide inhibitors
What are the three types of reversible inhibitions
Competitive, Uncompetitive, Non-Competitive
True or False, Km is decreased in the presence of a competitive inhibitor
False, it’s increases
True or False, maximal velocity is decreased in the presence of a competitive inhibitor
False it is constant
True or False, Non-competitive inhibition occurs when the inhibitor and the substrate binds to the same site on the enzyme
False, they bind on different sites
In non-competitive inhibition, Vmax is _______, do to the effects not being overcome by an increase in substrate concentration
Decreased
In non-competitive inhibition, Km is _________, due to the the inhibition not affecting the binding of the enzyme-substrate
Constant
Where does the inhibitor bind to in uncompetitive inhibition
Enzyme-substrate complex
Km and Vm __________ in uncompetitive inhibiton
Decreases
True or False, in non-competing inhibition, Inhibitor binds only to the enzyme
False, it binds to both enzyme and enzyme-substrate complex
In non-competitive inhibition, Vm ______ while Km ______
Decreased, remains the same
Allosteric Enzymes are regulated by ________
Effectors
What the names of the two binding sites for allosteric enzymes
Active and Allosteric
________ concentration affects allosteric enzymes
Substrate
Hb is an example of what type of non-michaelis menten kinetics
Cooperativity
What are the two states in a concerted model
Tense or relaxed
When a ligand binds, it drives the equilibrium towards what state in a concerted model
Relax
R concentration ________ apparent Km
Decreased
Inhibitor binds drives equilibrium to _______
T
T concentration increases _______
Km
What disease causes a 3-6 month baby to not be able to turn over, sit or crawl
Tay-Sachs Disease
What are the symptoms of Tay-Sachs disease
Seizures hearing loss, immobility
What enzyme is absent in Tay-Sachs disease
Beta-Hexosaminidase A
What is accumulated in Tay Sachs due to the absence of an enzyme
GM2 ganglioside
True or False, there is no cure for Tay-Sachs disease
True
What disease cause symptoms of intellectual disability, seizures and mental disorders
Phenylketonuria
What enzyme is absent to cause Phenylketonuria
Phenylalanine Hydroxylase
What disease is caused by a decrease of Phenylalanine
Phenylketonuria
What disease causes symptoms of severe gout, renal problems, poor muscle control
Lesch-Nyhan Syndrome
What enzyme is inhibited in Lesch Nyhan Syndrome
Hypoxanthine-guanine phosphoribosyltransferaee
What pathway is affected in Lesch Nyhan syndrome
Purine Salvage Pathway
What substance is built up in Lesch Nyhan syndrome
Uric Acid
True or False, there is no cure for Lesch-Nyhan Syndrome
True
What disease causes symptoms of Developmental Delay, Megaloblastic Anaemia, FTT
Hereditary Orotic Aciduria
What enzyme is inhibited in Hereditary Orotic Aciduria
Unridine Monophosphate Synthase
What pathway is affected in Hereditiry Orotic Aciduria
Pyrimidine - Urea cycle
What can be used to treat Hereditary Orotic Aciduria
Uridine Monophosphate/Uridine Triacetate
