Enzyme: Kinetics Flashcards
It lists the initial chemical species present and the new chemical species formed
Balanced chemical equation
It is used to describe reactions in living cells where the product of reaction are immediately consumed by subsequent enzyme catalyzed reaction
Unidirectional arrows
Describes both the direction in which a chemical reaction will tend to proceed and the concentration of reactant and products that will be present in equilibrium
Gibbs free energy change
_____ of the free energy change determines how far the reaction will proceed
Sign and Magnitude
G is independent of the _____ of the reaction and therefore provide no information concerning ____
Mechanism , rate
The natural and favorable tendency of process is to proceed from an area of
High energy to an area of low energy
Used to predict the direction of a reaction at a constant temperature Nd pressure
Standard free energy
Net loss of energy
Exergonic reaction
Net gain of energy
Endergonic reaction
Reaction in equilibrium
Equal
Spontaneous reaction
Exergonic reaction
Non spontaneous reaction
Enedergonic reaction
Forward reaction is equal to backward reaction
Reaction in equilibrium
Field of biochemistry concerned with the quantitative measurement of the rates of enzyme catalyzed reaction
Enzyme kinetics
Constant reaction and product
1 molar
Constant temperature
25C or 298 K
Constant temperature
1 atmosphere
It states that Standard free energy of all consecutive reactions in a pathway are additive
Coupling reaction or hess law
For 2 molecules to react, they must
Approach a bond forming distance
Possess enough kinetic energy to surpass energy barrier
Factors affecting collision
Substrate concentration
Temperature
pH
Inhibitors
It relates reaction rate to substrate concentration
Michaelis menten equation
Michaelis menten equation have _____ curve
Hyperbolic curve
Tell you how fast the reaction is
Vi or velocity
Michaelis constant is equal to
Half the maximal velocity
Maximal number of substrate molecules converted to product per unit time
Vmax
Rate is not affected by substrate
Above Km
Above Km
Zero kinetics
First order kinetics
Below Km
Rate directly proportional to substrate
Below Km
Reciprocal of michaelis menten eq.
Lineweaver burke plot
Used to calculate Km and Vmax
Lineweaver burke plot
It determines the mechanism of action of enzyme inhibitors
Lineweaver burke plot
Lineweaver burke plot is also called
Double reciprocals
It used for multimeric enzymes
Hill equation
Equation which show cooperativity
Hill equation
Increasing kinetic energy of molecules will…
Increase rapidity of motion
What are the factors affecting reaction rate?
Temperature
pH
Reactant concentration
Inhibitors
Any substance that diminishes the velocity of an enzyme catalyzed reaction
Enzyme inhibitor
Type of enzyme inhibition that bind to covalent bond
Irreversible
Type inhibition that competes on the same active site
Competitive inhibition
Inhibitor that binds on another site different from active site
Non competitive inhibition
Enzyme + substrate
Enzyme substrate complex
Competitive reversal
Increase of substrate
Non competitive reversal
Increase of enzyme
Km in competitive inhibition
Increases
Km in non competitive inhibition
The same since they don’t interfere with binding
Vmax of competitive inhibition
Not changed
Vmax of non competitive inhibition
Vmax lowered
