Enzyme Kinetics Flashcards
What is proteases ?
Needed for digestion, recycling of amino acids, activation of enzymes
What is chymotrypsin ?
covalent intermediate
Acid-base catalysis
Active residue is OH
Nucleophile
What does the serine OH group on enzyme attack and breaks ?
Peptide bond in proteins. Forming a covalent intermediate with the carboxyl fragment. Aminyl fragment released
What is the definition of energy ?
The term energy is the capacity to do work
What is bioenergetics ?
Describes how living systems capture, transform and use energy to perform work and stay alive
How do biochemists measure energy involved in metabolism ?
Mostly using equilibrium thermodynamics
Whats a system ?
Is defined as the matter within a defined region of space
Whats the first law of thermodynamics ?
Energy within a system is conserved, it can be changed from one form to another, but it cannot be created or destroyed.
What does the bomb calorimeter measure ?
Direct way of measuring the energy content of metabolites an food stuffs
In the bomb calorimeter what can the energy produced by combusting or oxidising a substrate be measured as ?
A change in internal energy.
What is the enthalpy of a system a measure of ?
The heat change during a reaction at constant pressure and volume.
In exothermic reactions is the change in energy positive or negative ?
change in energy is negative
Explain how the bomb calorimeter can help us calculate the energy produced in the reaction
GLUCOSE – LACTATE ?
- First combust a fixed quantity of glucose and measure change in enthalpy
- In separate reaction, combust a fixed quantity of lactic acid and measure change in enthalpy
C02 + H20 —— C02 + H20
Whats the efficiency equation ?
EFFICIENCY = ENERGY OUT / ENERGY IN X 100
Change in enthalpy involves changes in what ?
Useful and useless energy
