Enzyme Activity Flashcards
1
Q
cellobiase
A
- Enzyme that catalyzes the hydrolysis of glucose B 1-4 glycosidic bonds
- Crucial to life cycles of organisms that obtain energy by hydrolyzing cellulose
2
Q
cellulose/cellobiose
A
Most prominent structural carbohydrate of plants
3
Q
ethanol
A
fuel made by breaking down cellulose into glucose
4
Q
p-Nitrophenyl glucopryanoside (pNG)
A
- Artificial substrate
- Produces a colored product when exposed to a solution that is basic in pH.
- Absorbs light at the wavelength of 410 nm
5
Q
spectrophotometer
A
- Transmits beams of light through a substance to measure how much to the light is absorbed.
- The darker the color the more product is formed
- Rate of reaction can be measured
6
Q
Brownian motion
A
Randomly moving molecules
7
Q
Vmax
A
- All enzymes in solution are actively catalyzing the formation of product
- Add more enzymes to increase
8
Q
Environmental factors that effect enzymes
A
- Temperature
- pH
- Enzyme concentration
- Substrate concentration
