Enzyme

Question 1

Lock + key

Answer 1

Shape of substrate + enzyme active site are COMPLEMENTARY , enzyme is specific

Question 2

induced fit

Answer 2

Enzyme active site changes shape to ACCOMODATE substrate once substrate binds
- conformational change

Question 3

Effect of high temp

Answer 3

Increase kinetic energy , more successful collisions , more ESC complexes form. Too high temp - Faster initial rate then DENATURES ( so may not reacted w all substrate) , affects bonds in TERTIARY structure , changes shape of active site - prevents substrate binding
Low temp effects reversible - fewer successful collisions

Question 4

Things to improve experiment

Answer 4

-control PH, TEMP, CONC. , vol of solutions
- temp intervals closer tg more accurate estimate of OPTIMUM temp
Control group
Take samples at more frequent intervals
Use coloritometer
Use PH probe - numerical value

Question 5

Unit + formula flr rate

Answer 5

1/time
Mm 2/s-1

Question 6

Unpaired t test

Answer 6

Compares means of two data sets

Question 7

What does standard deviation show

Answer 7

Shows spread of data AROUND mean
Small SD- little variation in repeats , high repeatability
As conc increases, SD increases , repeatability decreases

Question 8

Enzyme conc effect

Answer 8

As increases , rate increases , MORE ESC formed, more active sites available , conc of substrate remain the same , rate levels off substrate conc is limiting

Question 9

Non- competitive inhibitor evfect

Answer 9

Bind to allosteric site, changes shape of active site , substrate can no longer bind to it

Question 10

Cofactor

Answer 10

Inorganic ion which helps
CL- for amylase
ZN2+ - carbonic anyhdrase

Question 11

Competitive inhibitor

Answer 11

Competes w substrate to bind to active site,binds to active site + prevents substrate from entering active site