Cell Structure Flashcards
Lysosomes
Contain hydrolysing enzymes
Digest pathogens
Cystoskeleton function
Maintain shape
Movement of cilia/ flagella
Support organelles keeping them IN PLACE
Movement of chromosomes when they separate during cell division (microtubulee in spindle)
Cytoskeleton
Network of protein threads
MicrOfilaments + microtubules
Protein pathway
Ribosomes on RER site of protein synthesis
Vesicles transport protein to Golgi apparatus - processes proteins eg adds carbohydrate chain
vesicles fuse to plasma membrane
Smooth endoplaskic reticulum
Processes + synthesises lipids
Light microscope resolution
LOW - 0.2 um
Why staining good
Easier to see more detail
Provides contrast
Identity organelles
Golgi apparatus
Rececives protein from RER/ ribosomes
Modify / processes / makes glycoproteins
Re package into vesicles
Make lysosymes
DNA in prokaryotic
Free in cytoplasm , circular
Plasmid
Smaller ribosomes also
Root hair cell adaption
Thin cell wall
Hairs - large SA
Carrier proteins - AT for ions
Many mitochondria
High Sa:V
Plant cell has but not animal
Chloroplast , cell wall, vacuole, plasmosdesma
Um to m
1mm = 1000um
Magnification
Image / actual size
Scanning electron microscope
Scan beam electrons across specimens
Middle magnicatjon + resolution
3D
TEM
Best resolution + magnification
Gram stain technique
Separate bacteria into gram POSITIVE + gram NEGATIVE crystal violet applied to bacterial specimen on slide then iodine , slide then washed w alcohol
Gram positive will retain crystal violet stain , appear purple/blue
Gram negative - thinner cell walls will lose stain , then stained w safrnanin dye , stains RED
Gram positive - susceptible to penicillin,inhibits formation of cell walls
Eye piece graticule + stage micrometer
Each division on stage micrometer is 0.1mm long
E.G 1 division on stage micrometer is same as 4.5 on eyepiece graticule , 1 division on graticule = 0.1 divided by 4.5
0.1 divide by no. Of eyepiece divisions = 1 division on eyepiece
Real size = eyepiece divisions times answer
