Enzymatic Reactions Flashcards
What is the pH practical
Put a drop of iodine solution into every well of a spotting tile
Place a Bunsen burner on a heat proof mat and a tripod and gauze over it.
Put beaker on top of tripod and heat water until it is 35*C
Use syringe to add 1cm(3) of amylase solution and 1cm(3) of a buffer solution with pH of 5 to boiling tube.
Using test tube holders put the tube into the beaker of water and wait for 5 mins
Use a different syringe to add 5cm(3) of starch solution to the boiling tube
Mix the contents of the tube and start a clock
Use continuous sampling to record how long it takes for the amylase to break down all of the starch by using a dropping pipette to take a fresh sample from the boiling tube every 30 seconds and drop into a well
When the iodine solution is browny orange starch isn’t present
Repeat the experiment with different pH values to see how pH affects the time taken for starch to break down
Control the variables to make it a fair test
What is the rate of reaction equation
Rate=1000/Time
What is the unit of Rate
S^-1
What is another way of calculating the rate
Amount it has change/Time taken
An enzyme controlled reaction was carried out at pH 4. After 60 seconds 33cm(3) of product has been released. Calculate the RoR in cm(3) s(-1)
33/60 = 0.55 cm(3) s(-1)
