Enterobacteriaceae Flashcards
Where do Enterobacteriaceae reside?
In GI tract of humans and animals
List 4 benefits of Enterobacteriaceae
- Microbial antagonism
- Breakdown and absorption of food
- Waste processing
- Vit. K production
List 4 characteristics of Enterobacteriaceae
- Gram negative
- Facultative anaerobic
- Rods or coccobacilli
- Oxidase negative (except Plesiomonas and shigelloids)
T/F: Enterobacteriaceae are coliform bacteria
True
What is performed to evaluate fecal/sewage contamination?
Coliform enumeration
List 3 characteristics of Escherichia coli
Lactose positive
Oxidase negative
Motile rods
What is the complete antigenic formula of an E. coli called?
Serotype
What do the following serotypes stand for?
O, H, F, K
O= O antigen H= Flagella F= Fimbriae K= Capsule
T/F: The pathogenicity of E. Coli is strain dependent
True
What two groups of infectious strains can E. coli have?
- Intestinal pathogenic strains (Enteric)
2. Extra-intestinal pathogenic strains (ExPEC)
What kind of infections can ExPEC strains of E. coli cause?
Respiratory
Septicemia
UTI
Mastitis
What are 4 cell associated virulence factors of E. coli?
- Endotoxin
- Capsule
- Fimbrial adhesions
- Non-fimbrial adhesions
What are 3 types of extracellular virulence factors E. coli can have?
- Enterotoxins
- Cytotoxins
- Siderophores
What are 5 enteric pathotypes of E. coli?
- ETEC (Enterotoxigenic)
- EHEC (Enterohemorrhagic)
- VTEC/STEC (Verotoxigenic/shiga-like toxic)
- EPEC (Enterpathogenic)
- RPEC (Rabbit pathogentic)
What are 4 extra-intestinal pathogenic E. coli?
- APEC (avian pathogenic)
- Necrotoxigenic (NTEC) in cattle
- Mastitis causing E. coli in cattle
- MMA in pigs
What are 5 important E. coli in pigs?
ETEC VTEC EPEC UTI MMA
What are 6 important E. coli in bovine?
ETEC EHEC VTEC EPEC Mastitis Septicaemiae
What is an important E. Coli strain in dogs and cats?
UTI
What is an important E. coli strain in birds?
APEC
What is an important E. coli strain in rabbits?
RPEC
What are 4 problems E. coli causes in pigs?
- Diarrhea
- Oedema disease
- UTI
- MMA
What does ETEC cause in pigs?
Watery diarrhea
Who does ETEC affect in pigs?
Neonatal, 2-4 weeks, weaning
What are the two virulence factors involved with in ETEC in pigs?
- Adhesion factors (small Intestine)
2. Enterotoxins (plasmids)
What are the 5 adhesion factors involved with ETEC in pigs?
F4 F5 F6 F41 F18
What are the 4 enterotoxins involved with ETEC in pigs?
- LT (heat labile)
- STa (heat stable)
- STb (heat stable)
- EAST1 (heat stable)
What are the Neonatal virulence factors of ETEC in pigs?
F4, F5, F6, F41
STa, STb
Non-hemolytic
What are the Neonatal till 4 weeks virulence factors of ETEC in pigs?
F4
LT, STa, STb, EAST1
Hemolytic
What are the weaning diarrhea virulence factors of ETEC in pigs?
F4, F18
LT, STa, STb, EAST1
Hemolytic
T/F: In regards to the epidemiology of ETEC in pigs, there is an equilibrium between maternal immunity and infection pressure
True
What distortion in equilibrium causes piglet diarrhea from ETEC?
Too high bacterial challenge and/or too low immunity (mainly maternal)
What are 4 pathogenesis factors of ETEC in pigs?
Ascending from lower bowel
Oral uptake
Small Intestine
Colonization
What are the symptoms seen with ETEC in pigs?
Remain drinking
Very smelly feces
Dehydration
White grey, not as watery diarrhea in older animals
How would ETEC in pigs be diagnosed?
Agglutination tests
PCR
What would be the treatment for ETEC in pigs?
Hydration
Antimicrobials
What would be the preventative measures form ETEC in pigs?
Hygiene
Vaccinate sows
Vaccinate piglets (for F4)
Selection for reception free piglets (F4)
What does VTEC in pigs cause?
Oedema disease post weaning
What does a mixed ETEC and VTEC disease in pigs cause?
Oedema disease and diarrhea
What are the 3 virulence factors of VTEC in pigs?
Adhesion (small bowel F18)
Exotoxin (VT2e or Stx2e: media necrosis)
Endotoxin (acute mortality)
How is VTEC passed in pigs?
Sow to piglet
What are some reasons VTEC shows up at weaning in piglets?
Stress
Change in intestinal flora
Maternal immunity
What does hyper-acute toxin production of VTEC cause in pigs?
Enterorrhagia
What does acute toxin production of VTEC in pigs cause?
Permeability blood vessels odema
What does a slower toxin production of VTEC in pigs cause?
Intravascular coagulation
What does endotoxin release of VTEC in pigs cause?
Shock and acute mortality
When do symptoms start being seen in pigs with VTEC?
3 weeks after weaning
What are the clinical signs of VTEC in pigs?
Acute mortality
Diarrhea
Anorexia
Oedema
What is seen with oedema?
Hoarse voice
CNS signs
Dyspnoea
Eye lids swollen
What would you do to diagnose VTEC in pigs?
CS
Pathological findings
Bacteriology
PCR (demonstration of virulence factors)
How would you treat affected pigs with VTEC?
Fasting to eliminate toxin
Antimicrobial therapy
How would you treat pigs not yet affected with VTEC?
Antimicrobial therapy
T/F: Antimicrobial therapy will kill the bacteria and effective toxin
False; will not kill effective toxin therefore will still see symptoms after treatment
What are some preventative measures against VTEC in pigs?
Reduce stress
Organic acids
Zinc oxide
Selection form receptor F18 negative animals
T/F: EPEC in pigs is a common disease
False, more rare and little known about its general role
What age does EPEC in pigs effect?
Weaning piglets
What are the CS of EPEC in pigs?
Diarrhea
How would a diagnosis be accomplished for EPEC in pigs?
Isolation and PCR of eae gene
What are the 3 pathogenic intestinal E. coli in piglets?
ETEC
VTEC
EPEC
What are the virulence factors of EPEC in pigs?
Attaching and effacing
Eae gene
T3SS (needle) and injection of effector proteins
What is the most important pathogen of UTI in pigs?
UTI (DD actinobaculum suis)
What are the predisposing factors for UTI in pigs?
Hygiene Water intake Obstipation (severe constipation) Age Individual differences
What are the CS of UTI in pigs?
Anorexia
Hematuria
Vaginal exudate
General malaise
How would a diagnosis be made for UTI in pigs?
Bacteriolgy
What is the treatment and prevention plan with UTI in pigs?
Culling (fertility problems)
Antibiotics about 3 weeks
What is MMA or PPDS in pigs?
Metritis-mastitis-agalactiae
Post partum dysgalactiae syndrome
When does MMA affect pigs?
About 48 hours after farrowing (parturition)
What is the etiology of MMA in pigs?
Hereditary
Hormonal
Feed
Infectious agents (E. coli mainly, might find others)
What kind of infection does septicaemiae cause in pigs?
Secondary infection to enteric infection
Primarily rare
What are the E. coli disease in bovine?
ETEC
EPEC and EHEC
Septicaemic (NTEC)
What are the symptoms of ETEC in bovine?
Watery diarrhea leads to dehydration
What age group does ETEC affect in bovine?
Animals less than 3 days old, Neonate
What are the virulence factors of ETEC in bovine?
Fimbriae
Toxins
What are the Fimbriae virulence factors of ETEC in pigs?
F5 (most common)
F41 (frequently together with F5)
F17
CS31A (related to F4/diarrhea in older animals)
What are the toxins for ETEC in bovine?
Sta and Stb (heat stable toxins)
What is the pathogenesis of ETEC in pigs?
Oral uptake
Multiplication
Enterotoxin production
Secondary septicaemiae
T/F: There is an equilibrium between immunity and infection pressure of ETEC in bovine
True
How does colostrum and infection play a role in ETEC of bovine?
If colostrum is given too late, or there is too little or too low (in antibodies)
If infection occurs too early or it is it too high of infection pressure
How is ETEC in bovine diagnosed?
ELISA
Culture and virulence factors (agglutination and PCR)
What is the therapy plan for ETEC in bovine for sick animals?
Separate ill animals
Hydration
Antibiotics
NSAID (shock)
What is the treatment plan for milking farms affected by ETEC for bovine?
Hygiene (separate calves from mothers)
Colostrum
Antibiotics 4-5 days orally
Vaccination of dams
T/F: EHEC in bovine is zoonotic
True
What age group does EHEC and EPEC of bovine affect?
1-8 weeks
What are the CS of EHEC and EPEC in bovine?
Mucoid diarrhea +/- blood
Mainly in large intestine
What is there therapy plan for Tx of EHEC and EPEC in bovine?
Ab’s
What are the virulence factors for EHEC and EPEC in bovine?
EPEC = eae
EHEC = eae + VT (VT1 and VT2)
What are the CS of mastitis in bovine?
Udder swelling, redness, and pain
What is the prevention and Tx plan for mastitis in bovine?
Hygiene
Systemic antibiotics
T/F: Mastitis of bovine has known virulence factors
False, no know virulence factors
What kind of pathogen is septicaemiae (NTEC) in bovine?
Facultative pathogen
What are the virulence factors of septicaemiae (NTEC) in bovine?
Serum resistance
Iron uptake systems
Endotoxin, capsule
Adhesion factors
What are the adhesion factors of septicaemiae (NTEC) in bovine?
P F17 CS31A AfaE-VIII CNF1, CNF2 toxins
What are the two ways of uptake for septicaemiae (NTEC) in bovine?
Per os
Umbilical cord
What is the PO route of septicaemiae (NTEC) in bovine?
PO -> Lymph tissue -> septicaemiae colostrum
What is the umbilical chord route of septicaemiae (NTEC) in bovine?
Umbilical chord -> septicaemiae colostrum
What are the hyperacute signs of septicaemiae (NTEC) in bovine?
Acute mortality
Shock
What are the acute CS of septicaemiae (NTEC) in bovine?
General symptoms
Fast Mortality
What are the CS of a slower septicaemiae (NTEC) in bovine?
Association with organ localization Poly-arthritis Pneumonia Pleuritis Me I go-encephalitis
How would septicaemiae (NTEC) in bovine be diagnosed?
Clinical signs
Isolation + PCR
What is the therapy plan for septicaemiae (NTEC) in bovine?
NSAID (shock)
AB’s
Gamma globulins
How would septicaemiae (NTEC) in bovine be prevented?
Colostrum
Which strains are associated with enteric disease in cats and dogs?
ETEC
EPEC
VTEC
EIEC
What is the virulence factors of enteric disease in dogs and cats?
CNF1 E. coli in pups