Enterobacteriaceae Flashcards
His antigenic structure is mainly composed of repetitive polysaccharide units
O antigen
This antigenic structure interferes with O antisera
K antigen
This is the most external part of the cell wall LPS
Somatic antigen
This antigen reacts with IgG
Flagellar antigen
Among the 3 antigen, which is heat stable
O antigen
This antigen is composed of either protein or polysachharides
Capsular antigen
This is associated with virulence
Capsular antigen
They are preserved with formalin and reacts with IgG
Flagellar antigen
This group mainly ferments and do not oxidize. True or false
True
They are both catalase and oxidase positive.
False. Oxidase negative
Cannot oxidize
Describe.
3M FAzza
3M FAzza
Most pathogenic
Most motile
Most often encountered
Fac. Anaerobe
They are motile because of their peritrichious flagella. Except?
SK - mabagal
Shigella
Klebsiella
True pathogens are
YESS
Yersinia
E coli
Shigella
Salmonella
This type of toxin has specific effect on host cell
Exotoxin
Mainly protein based
Exo
Lipid A is a toxic component
Endo
Mainly LPS based
Endo
Varied effect
Endo
Produced only by gram negative
Endo
State the fast slow and non LF
EEK
CHASE
PSS
This culture contains bile salts and crystal violet which inhibita growth of gram positive and fastidious nega
MacConkey
What is the result in MacConkey for non LF and LF
Non LF - Transparent
LF - red colonies
In EMB agar what is present that inhibit the growth of gram positive and fastidious negative
Aniline dyes
Eosin and methylene blue
EMB RESULT or non lf and lf
non lf - transparent
Lf - green black with metallic sheen
Indicator of acid prodxn in emb agar
Anilin dyes combine to form a precipitate
This is the only agar that can differentiate nonlf
Emb agar
This agar also has bile salts and acid indicators such as bromthymol blue and acid fuschin
Hektoen agar
This agar is useful in identifying potential pathogen from normal flora in stool
Hektoen agar
Result of hektoen
Non lf - green to blue green
Lf - orange
Characterize E coli
Smooth Motile Flat Non viscous Metallic sheen
Non LF that is non motile, no gas formed
Shigella
Non LF, swarming on agar. Hydrolyses urea rapidly
Proteus
LF, non motile, very mucoid and viscous
Klebsiella
Rapid LF, motile, has mo metallic sheen yet mucoid
Enterobacter
Positive on urease test
Proteus
Klebsiella
P-u-K
Positive on SIM
- indole - proteus, e coli
- motility - pees me
Positive on citrate or simmon test
CSK
CITRO
SALMO
KLEB