Energy Transfers In And Between Organisms Flashcards
What is Photosynthesis?
- Reaction in which light energy is used to produce glucose in plants.
- Water + Carbon Dioxide = Glucose + Oxygen
What are the two stages of Photosynthesis?
- Light dependent reaction
- Light independent reaction
What is the rate of Photosynthesis determined by?
- Carbon dioxide concentration, light intensity and temperature.
What ways are chloroplasts adapted for Photosynthesis?
- Stacks of Thylakoid membranes called Grana = large surface area for the attachment of chlorophyll, electrons and enzymes.
- Network of proteins = hold the chlorophyll in a very specific manner = absorb maximum amount of light.
- ATP synthase channels = selectively permeable = proton gradient = synthesis of ATP.
- DNA and Ribosomes = synthesis proteins for the light dependent reaction.
Light Dependent reaction = What is Photoionsation?
(Non-cyclic Photophosphorlation)
- Photons of light are absorbed by PSII
- Energy excites two electrons to a higher energy level
- 2e- move along the e- transfer chain to PSI
Light Dependent reaction = What is Photolysis?
(Non-cyclic Photophosphorlation)
- The splitting of water with light
- H20 -> 2H+ + 0.5o2 + 2e-
- One molecule of water = 4 photons to split
- Electrons produced replace lost electrons in PSII
- Protons move into stroma creating a proton gradient
Light Dependent reaction = How does energy from electrons produce ATP?
(Non-cyclic Photophosphorlation)
- Excited electrons move along electron transfer chain = loosing energy.
- Lost energy provide energy for active transport of H+ ions from a low to high gradient into the Thylakoid space from the stroma across the proteins = proton pump created.
- High concentration of H+ ions in thylakoid space allows a facilitated diffusion through ATP synthase into the stroma.
- Energy from this combines ADP and Pi to from ATP.
Light Dependent reaction = How is reduced NADP made from energy from the electrons?
(Non-cyclic Photophosphorlation)
- Light energy is absorbed by PSI
- 2e- becomes excited and leaves PSI to form NADP
- Protons int eh stroma combine with NADP or make reduced NADP.
Light Dependent reaction = Cyclic Photophosphrolation
- Light energy excites 2e- in PSI, move down electron transfer chain creating proton gradient and energy for active transport of H+ ions into Thylakoid membrane.
- ATP synthase makes ATP, electrons return to PSI.
What is the Chemiosmotic theory?
Process of electrons flowing down the electron transfer chain to create a proton gradient to drive ATP synthesis.
Light Independent reaction = What happens in Carbon dioxide fixation/stage 1?
- CO2 diffuses through the stomata fixed with ribulose bisphosphate (RuBP) = carboxylation.
- Enzyme Rubisco needed.
- 6 carbon sugar is formed first, very unstable so it forms 2 molecules of glycerate-3-phosphate.
Light Independent reaction = What happens in the Reduction phase/stage 2?
- The 2 molecules of glycerate-3-phosphate contain a -COOH group = acid.
- Reduced NADP from the light dependent reaction then reduced the glycerate-3-phosphate, with energy provided from ATP.
- Forms 2 molecules of triose phosphate
Light Independent reaction = What happens in the Regeneration of RuBP phase/stage 3?
- 5 molecules of triose phosphate are used in order to regenerate 3 molecules of ribulose bisphosphate.
- Remaining ATP from light dependent reaction is used to do this.
- The other 1 molecule of triose phosphate goes towards making glucose.
Light Independent reaction = What happens int eh Organic molecule production/stage 4?
- 2 molecules of triose phosphate can combine to form the intermediate hexose sugar (fructose 1,6 bisphosphate)
- 6 turns of the Calvin Cycle needed in order to produce one molecule of glucose per molecule of CO2.
What is the Lollipop experiment - Melvin Calvin?
- Radioactive carbon added to algae to be incorporated during photosynthesis.
- After a set period of time, algae is killed in alcohol to prevent further reaction,
What is a limiting factor?
In photosynthesis?
- A factor that decreases of limits the rate of Photosynthesis
- Rate of physiological process will be limited by the factor which is in shortest supply.
What is the Compensation point?
- When carbon dioxide released during respiration is equal to that taken up by Photosynthesis.
How does light affect the rate of Photosynthesis?
- As light intensity increases = rate of photosynthesis increases
- More Photons of light = greater the number of chlorophyll molecules are ionised and more ATP and NADPH are generated.
- Will be limited by some other factor
- Very very high light intensity = chlorophyll may become damaged
How does Carbon dioxide affect the rate of Physical?
- As CO2 increases = rate of Photosynthesis increases
- Plateaus when the maximum rate of fixation is reached
How does temperature affect the rate of Photosynthesis?
- Light independent reactions = DEPENDENT of temperature = reaction is catalysed by enzymes
- As temperature increases up to the optimum temp = rate of Photosynthesis increases
- Aprox. Doubles for every 10’C
- Aboce optimum temperature rate decreases as enzymes denature
What are the four stages of respiration?
1) Glycolysis - conversion of glucose (6C) to pyruvate (3C) (anaerobic)
2) Link reaction - Pyruvate is converted yo acetyl-coenzyme A (2C)
3) Krebs cycle - cycle of oxidation and reduction reactions that yield ATP and reduced NAD and reduced FAD
4) Oxidative phosphorylation- electron transfer chain using reduced NAD and FAD to from ATP and water
Where does each stage happen?
1) Glycolysis = cytoplasm of cell
2) Link reaction = matrix of mitochondria
3) Krebs cycle = matrix of the mitochondria
4) Electron transfer chain = proteins of the membrane of the crista
What happens in glycolysis?
- ATP adds one phosphate group to a glucose molecule = glucose phosphate (6C)
- Another phosphate group is added to glucose phosphate to form hexose bisphosphate (6C) - requires energy from ATP
- Hexose bisphosphate (6C) is then broken down into 2 molecules of triose phosphate (3C)
- Each triose phosphate is oxidised by NAD to form reduced NAD - 2 molecules of reduced NAD from 1glucose
- Each molecule of triose phosphate (3C) is converted to pyruvate (3C) - due to the removal of both phosphate groups by ADP to form ATP - each triose phosphate form 2 ATP
- If oxygen present - pyruvate transfers from the cytoplasm to the matrix of the mitochondria for the link reaction and Krebs cycle
- If oxygen not present - pyruvate is converted to lactate or ethanol and CO2 in anaerobic respiration
What are the main products of Glycolysis?
- 2 reduced NAD (NADH + H+)
- 2 Pyruvate
- 2 ATP (net)