Energy And Respiration Flashcards

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1
Q

Why do living organisms need energy?

A

To Work!
Examples of work:
Anabolic Reactions for example protein synthesis, synthesis of glycogen (glycogenesis), DNA replication, polymerisation.
Active transport: for example Na+-K+ pump, movement of vesicles in exo/endocytosis.
Movement: For example muscle contraction, cilia/flagella, movement of chromosomes
Maintenance of a constant body temperature in warm blooded animals for example endotherms.
Bioluminescence/electrical discharge for example in jellyfish and electric eels.

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2
Q

What is the meaning of anabolic reactions?

A

Anabolic reactions is the building of large molecules from smaller molecules using energy.

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3
Q

What is the meaning of catabolic reactions?

A

Catabolic reaction is when a large molecule is broken down into smaller molecules and releases energy.

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4
Q

Describe the structure of ATP

A

Adenosine triphosaphate
It is a phosphorylated nucleotide.

Its structure has three components.
1) Adenine (organic, nitrogenous base)
2)Ribose sugar (Pentose sugar)
3)Three phosphate groups

1+2=adenosine (nucleoside)

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5
Q

What are the chemical properties/characteristics of ATP?

A
  • Small
  • Water-Soluble
    →Easily transported around the cell
  • Readily hydrolysed/lose phosphate to release energy
  • Small packets of energy released at one time.
  • ATP can be synthesised and broken down quickly
    →High turnover rate

This makes it ideal as an energy currency in all organisms.

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6
Q

What is the roles/functions of ATP as a universal link/intermediate energy molecule:

A

a)The universal link/Intermediate energy molecule
* Between energy-giving reactions and energy-requiring reactions.
*Examples of energy-giving reactions: aerobic respiration aka complete oxidation of glucose.
→Gives a very large quantity of energy 2870KJ per molecule.
C6H12O6+6O2→6CO2+6H2O+2870KJ
Hydrolysis of ATP→Energy
* But energy transfers are inefficient
→Excess energy is lost at different stages in the multi-step reaction.
→As thermal/heat energy

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7
Q

What is the roles/functions of ATP as the energy currency of the cell:

A

b)ATP is the energy currency of the cell
* Energy giving/yeilding-reactions are linked to the production of ATP first,then energy requiring reactions.

ATP is NOT an energy storage molecule

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8
Q

What is an energy storage molecule:

A

An energy storage molecule stores energy in the form of chemical potential energy. For example:
Short term-Glucose,Sucrose
Long term-Glycogen,starch,triglyceride

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9
Q

What is the role of ATP as an immediate donor of energy?

A

ATP is an immediate donor of energy to reactions requiring energy.
Chemical equation of ATP hydrolysis:
ATP⇌ADP+Pi+30.5KJ
Where Pi is inorganic phosphate or
ATP+H2O ⇌ ADP + H3PO4 + 30.5KJ
Reactions are reversible
ATP can be synthesized and broken down quickly
Rate of turnover/interconversion of ATP is high
For example at rest we use about 40kg of ATP in 24 hours
Removal of 1st phosphate group ATP→ADP
→30.5KJmol-1 energy released
Removal of 2nd phosphate group from ADP→AMP
→30.5KJmol-1 energy released
Removal of the last phosphate group from AMP→Adenosine
14.2 KJmol-1 energy released

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10
Q

Where is ATP synthesised from?

A

ATP is synthesized from energy yielding reactions.
For example the oxidation of glucose and cellular respiration or light dependent stage in photosynthesis.
In a series of reactions.

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11
Q

What is respiration?

A

Respiration is a process where organic molecules such as glucose amino acid glycerol or fatty acids are broken down in a series of stages.
To release energy.
Which is used to synthesize ATP.
The two types are
1) aerobic respiration
2) anaerobic respiration

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12
Q

What is aerobic respiration and what are the four stages and their location?

A

Aerobic respiration is the breakdown of organic molecules for example glucose, fatty acids, glycerol and amino acids
to release energy
which is used to synthesize ATP
in the presence of oxygen.
There are four stages in aerobic respiration of glucose:
1) Glycolysis-Cytoplasm
2)Link Reaction-Mitochondrial matrix
3)Krebs Cycle-Mitochondrial matrix
4) Oxidative Phosphorylation-Inner mitochondrial membrane/cristae

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13
Q

Why is the oxidation of glucose in cellular respiration a multi-step reaction?

A

Each step releases a small quantity of energy(ATP) to:
1) Allow precise control
2)Cells could not fully harness total energy released if all were made available in one instant.
Also, reactions do not happen easily:
because glucose is a stable substance
It requires a high activation energy for the reaction to take place.
To overcome this:
1) Usage of enzymes to lower activation energy
2)Raising energy level of glucose by phosphorylation
➝more reactive

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14
Q

What are the steps in glycolysis or the glycolytic pathway?

A

1)Glucose (6C) is phosphorylated (phosphorylation)
*By 2 ATP
*Form hexose/fructose diphosphate (6C)
*This raises chemical potential energy of glucose
*Provide activation energy for split
2)Fructose diphosphate breaks down to 2 triose phosphate (3C) (lysis)
*6C➝2x3C
3) 2 hydrogen atoms are removed
*2 reduced NAD formed
*This is a dehydrogenation/oxidation reaction
4) 4 ATP produced (ATP is synthesized through substrate-level phosphorylation)
*4 ATP-2 ATP=net gain of 2 ATP
*Chemical potential energy is released from intermediate steps.
5) 2 pyruvate (3C) produced
Initial reactants:
1 Glucose (6C)
2 ATP
4 ADP, 2 NAD
Final products per molecule of glucose:
*2 pyruvate (3C)
*2 NADH
*4 ATP➝But 4-2=net gain of 2 ATP only!
The whole process involves many enzymes at each step

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15
Q

What are the different types of hydrogen acceptor molecules?

A

They are also called hydrogen carrier molecules:
You will learn 3 types:
1. NAD-nicotinamide adenine dinucleotide (used in respiration)
2. NADP-nicotinamide adenine dinucleotide phosphate) used in photosynthesis)
3. FAD-Flavin adenine dinucleotide (used in respiration)

They are all coenzymes= a non protein complex organic substance that is required for an enzyme’s activity.

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16
Q

Describe the structure of NAD:

A

*Nictonimide Adenine Dinucleotide
*Coenzyme
*H carrier molecule in respiration
Structure:
*Two linked nucleotides
*Both have ribose sugar and a phosphate group each
* 1 has an adenine base, the other nicotinamide ring
*Nictonimade ring-Accepts H
NAD+H⇋Reduced NAD
NAD+ + H+ +2e- ⇋ NADH

17
Q

What is the function of NAD?

A

The function of NAD is:
H Carrier molecule in respiration
Carry hydrogens from all stages of respiration (stage 1 2 3)
To take part in oxidative phosphorylation (stage 4) where most ATP is synthesized.

18
Q

Describe the structure of NADP

A

Nicotinamide Adenine dinucleotide phosphate
Coenzyme
Hydrogen carrier molecule used in photosynthesis
Different from of NAD
Structure:
Similar to NAD
But has a phosphate group instead of H on carbon 2 on ribose ring with adenine

19
Q

Describe the structure of hydrogen carrier molecule FAD:

A

Flavin Adenine Dinucleotide
Coenzyme
H carrier molecule in respiration
Used to carry H produced in Krebs cycle (stage 3 only)
→Then used in oxidative phosphorylation (stage 4)
Structure:
Two linked nucleotides
One nucleotide with phosphate, ribose and adenine
Another nucleotide with phosphate, ribitol and flavin.

20
Q

What are the steps in the link reaction?

A

Occurs in the mitochondrial matrix
*Pyruvate (3C) is transported from the cytoplasm
*Into the mitochondria matrix
*When oxygen is available
*By active transport
When oxygen is available, pyruvate (3C) is
1) Decarboxylated
→To form carbon dioxide
2) Dehydrogenated
→NADH produced
3)Combined with coenzyme A (CoA)
→ To form acetyl coenzyme A (2C)

21
Q

What is coenzyme A?

A

*Complex molecule
*Made of nucleoside (adenine+ribose) and a vitamin B5 (pantothenic acid)
Function
*Carry acetyl groups (2C) to Krebs cycle (stage 3)

22
Q

What are the steps in the Krebs cycle?

A

The Krebs cycle aka citric acid cycle/tricarboxylic acid cycle
Occurs in the mitochondrial matrix
* Enzyme-controlled pathway
1) Acetyle coenzyme A (2C)
→Combines with oxaloacetate (4C)
→To form citrate (6C)
→CoA removed and can be used again in link reaction (stage 2)
2) Citrate goes through a series of dehydrogenation and decarboxylation
→ 7 steps, by products of each step are
1. Nothing
2. Reduced NAD and CO2
3. Reduced NAD and CO2
4. ATP (substrate-level phosphorylation)
5. Reduced FAD
6. Nothing
7. Reduced NAD
3) Oxaloacetate (4C) regenerated
→ Can combine with another acetyl CoA
→Kreb cycle continues

23
Q

What are the final products per molecule of acetate CoA and per molecule of glucose.

A

The initial reactants are:
*1 Acetyle CoA (2C)
*1 Oxaloacetate (4C)
*1 ADP, 3 NAD and 1 FAD
Final products per molecule of acetyl CoA:
(this is 1 turn of the Krebs cycle)
* 3 NADH
* 1 FADH2
* 2 CO2 →waste gas, released
* 1 ATP
* Oxaloacetate (4C) → regenerated
But since 1 molecule of glucose (6C) oxidised into 2 pyruvate, which is converted to 2 acetyl CoA in the link reaction.
So everything is double in quantity with oxaloacetate (4C) being regenerated twice.

24
Q

Where does oxidative phosphorylation occur?

A

Occurs in the inner membranes/cristae of mitochondria
* High release of electrical potential energy here
→For the production of ATP (ADP+Pi→ATP)
*Involves a chain of electron carrier molecules in the inner membrane
→Electron transport chain (ETC)
*ATP synthesis is catalyzed by ATP synthase

25
Q

What is the first step in oxidative phosphorylation?

A

1) Hydrogen atoms removed from reduced NAD and FAD
* NADH and FADH2 are from glycolysis, link reaction, krebs cycle
* At the inner mitochondrial membrane
*Through dehydrogenation/oxidation reaction
*Catalysed by dehydrogenase enzymes
*NAD and FAD regenerated
→Can be reduced again in glycolysis, link reaction, Krebs cycle
Reduced NAD→NAD+H
Reduced FAD→FAD+2H
*Hydrogen atoms split into electrons and protons (H+ ions)
H→H+ + e-

26
Q

What is the second stage of oxidative phosphorylation?

A

2)Electrons are passed along Electron transport chain
*Passed along a series of electron carriers in inner membranes
*Electron carriers are associated with 4 types of membrane proteins
→Forms a functional unit called a respiratory complex
* Energetic electrons release energy as they pass through the ETC

27
Q

What is the third stage in oxidative phosphorylation?

A

3) Energy released is used to pump protons
*Across inner mitochondrial membrane
*From mitochondrial matrix to intermembrane space
* Since inner membrane is impermeable to protons
* And there is high [H+] in intermembrane space
→Proton gradient formed/electrochemical gradient formed

28
Q

What is the fourth stage of oxidative phosphorylation?

A

4) Protons move down electrochemical gradient
*By facilitated diffusion through ATP synthase
*Back into the mitochondrial matrix
*This provides energy for ATP synthesis
*This process is called chemiosomosis
*ATP synthase enzyme rotates
ADP+Pi→ATP
*ATP is synthesised
*Movement of 3 H+ ions back into matrix = 1 ATP molecule

29
Q
A