Cell Structure Flashcards

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1
Q

What is a cell?

A

A cell is the basic unit of all living organisms. It is surrounded by a cell surface membrane and contains genetic material (DNA) and cytoplasm containing organelles.

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2
Q

What is an organelle?

A

A functionally and structurally distinct part of the cell, e.g. a ribosome or mitochondrion.

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3
Q

What is a nucleus?

A

A nucleus is a relatively large organelle found in eukaryotic cells but absent from prokaryotic cells. The nucleus contains the cells DNA and therefore controls the activities of the cell. It is surrounded by two membranes which together form the nuclear envelope. As DNA is in the nucleus it is protected from degradation from enzymes.

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4
Q

What is a eukaryote?

A

Eukaryote is an organism whose cell contains a nucleus and other membrane-bound organelles.

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5
Q

What is a prokaryote?

A

An organism whose cells do not contain a nucleus or any other membrane-bound organelles.

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6
Q

What are the two types of microscopes?

A

There are two fundamentally different types of microscope: the light microscope and the electron microscope.

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7
Q

Which form of radiation do electron microscopes use?

A

The electron microscope uses electrons as a source of radiation.

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8
Q

Which form of radiation do light microscopes use?

A

The light microscope uses light as a source of radiation.

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9
Q

Describe the unit millimeter.

A

Millimeter (mm) is 10-3.

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10
Q

Describe the unit micrometer.

A

Micrometer (μm) is 10-6.

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11
Q

Describe the unit nanometer.

A

nanometer (nm) is 10-9.

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12
Q

Define the cell surface membrane.

A

Sometimes called plasma membrane. A very thin membrane (about 7nm in diameter) surrounding all cells. It is partially permeable and controls the exchange of materials between the cell and its environment.

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13
Q

Define chromatin.

A

The material of which chromosomes are made, consisting of RNA, visible as patches or fibers within the nucleus when stained. Chromatin=DNA and its associated proteins.

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14
Q

Define chromosomes.

A

Chromosomes are in the nucleus of the cells of eukaryotes, a structure made of tightly coiled chromatin (DNA, proteins and RNA) visible during cell division. The term circular DNA is now also commonly used for the circular strand of DNA present in prokaryotic cells.

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15
Q

Define nucleolus.

A

A small structure, one or more of which is found inside the nucleus. The nucleolus is usually visible as a densely stained body (densest region). Its function is to manufacture ribosomes using the information in its own DNA. Site of ribsomal RNA (rRNA) synthesis.

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16
Q

Define protoplasm.

A

All the living material inside a cell (cytoplasm+nucleus).

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17
Q

Define cytoplasm.

A

The contents of a cell, excluding the nucleus.

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18
Q

Define mitochondrion.

A

Mitochondrion is the organelle in eukaryotes in which aerobic respiration takes place. It is the most numerous organelle seen with a light microscope.

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19
Q

Define cell wall

A

A wall surrounding prokaryotes, plant and fungal cells. the wall contains a strengthening carbohydrate material cellulose which protects the cell from mechanical damage, supports it and prevents it from bursting by osmosis if the cell is surrounded by a solution with a higher water potential.
Thick rigid layer
Cell wall is freely permeable because there are spaces/gaps between fibres.
Cell wall limits cell size.

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20
Q

What is the average size of a bacteria cell?

A

An average bacteria cell is about 1 µm across.

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21
Q

What is the size of a ribosome?

A

One of the smallest structures you will study in the syllabus is a ribosome which is only about 25nm in diameter.

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22
Q

What is a photomicrograph?

A

A photomicrograph is a photograph of a specimen as seen with a light microscope.

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23
Q

Why are plant cells sometimes not stained?

A

The plant cells have not been stained because the chloroplast contain the green pigment chlorophyll and are easily visible without staining.

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24
Q

What is plasmodesma?

A

Plasmodesma (plural plasmodesmata) are a pore-like structure found in plant cell walls. Plasmodesmata of neighboring plant cells line up to form tube-like pores through cell walls, allowing the controlled passage of materials from one cell to the other. The pores contain ER and are lined with the cell surface membrane.
Plasmodesmata are strands of cytoplasm passing through channels. They contain little to no cell wall.
Function: allow substances to pass from cell to cell without passing through cell walls.
Plasmodesmeta are small in size so do not allow large organelles to pass through.

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25
Q

Define a vacuole.

A

An oraganelle found in eukaryotic cells, a large permanent central vacuole is a typical feature of plant cells, where it has a variety functions, including storage of biochemicals such as salts, sugars and waste products. Temporary vacuoles such as phagocytic vacuoles may form in the animal cells.
It pushes chloroplasts to the edge of cells so that chloroplasts are in a better position to capture sunlight.

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25
Q

Define tonoplasts.

A

The partially permeable membrane that surrounds plant vacuoles. It is also made up of phospholipid bilayer.

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26
Q

Define chloroplasts.

A

An organism bounded by an envelope (i.e. two membranes), in which photosynthesis takes in eukaryotes. Found mainly in the leaves. They are relatively large so can easily be seen with a light microscope.

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27
Q

Define photosynthesis.

A

The production of organic substances from inorganic ones, using energy from light.

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28
Q

Define Granum.

A

Grana (singular:granum) stacks of membrane inside a chloroplast. It is possible to see grana with a light microscope. These are the parts of the plants that contain chlorophyll the green pigment which absorbs light during the process of photosynthesis.

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29
Q

Define magnification.

A

The number of times larger an image of an object is than the real size of the object.
Magnification=Image size/Actual (real) size of the object.
1. Convert
2. Substitute
3. Calculate

magnification should always be whole number and x sign at front.

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30
Q

Which unit should actual size be usually expressed in light microscopes and electron microscopes?

A

We usually express the actual size of light microscopes in micrometer.
We usually express the actual size of electron microscopes in nanometer.
NOTE: always measure image size in mm (this can have marks).

31
Q

What components are needed to measure cells?

A

The stage micrometer which shows the true value of length (usually 10mm in length with 100 small divisions) appears bigger when magnification is increased.
The Eyepeice Graticule: Shows 100 graticule units which are in arbitary units. Appear constant no matter the magnification.

32
Q

What are the steps in measuring cells?

A
  1. Calibarate the EPG with the stage micrometer.
  2. Use the EPG to measure cell
33
Q

Descibe the nucleur envelope.

A

Made up of 2 membranes and is attached to the ER. It contains nucleur pores which controls movement of subtances between nucleus and cytoplasm.

34
Q

Describe ribosomes.

A
  • Smallest Organelle! (25nm)
  • Not bound by a membrane
  • Made of rRNA,that is synthesized in nuleolus+some protein.
  • Has 2 subunits.
    Function:
  • Site of protein synthesis.
    There are two types of ribosomes,
35
Q

Describe the two types of ribsomes.

A

80s ribsomes are 25nm. They are larger and found in cytoplasm and RER of all eukaryotes.
70S ribsomes are smaller and are found in mitochondria and chloroplasts of eukaryotes. Found in all prokaryotes.

36
Q

Describe the rough endoplasmic reticulum.

A
  • Extensive, connected system of membranes.
  • Made of cisternae (flattened membrane stacks)
  • Running through the cytoplasm
  • 80s ribsomes are attached,
37
Q

What are the functions of the rer?

A
  • Site of protein sythesis.
  • Protein Modification for example protein folding or glycosylation which is addition of carbohydate chains to protein.
  • Protein transport to golgi.
38
Q

Describe the smooth endoplasmic reticulum.

A

It is continuous with the rough endoplasmic reticulum and is the ER without ribsomes.MCQ: Membranes which surround an inclosed inner cavity.

39
Q

What is the function of the smooth endoplasmic reticulum?

A

Site of lipid and steroid sythensis for e.g cholestrol and steroid hormones.

40
Q

Describe golgi body

A

Also known as golgi apparatus/ complex
* Made of cisternae
* Have layered appeareance
* No connection between members
* Not continiuous with nucleur envelope
* Swelliings at end of sacs for vesicle formation
* constantly being formed and broken down.

41
Q

What is golgi body constantly being formed by?

A

Golgi body is constantly being formed by transport vesicles from RER on cis face.

42
Q

What is golgi body constantly being broken to form?

A

Golgi body is constantly being broken down to form secretory vesicles and lysosomes on trans face.

43
Q

List the structures involved in the production and secretion of proteins

A

1.Synthesis of proteins at ribosomes/ RER.
2. Transport vesicle buds off RER and fuses with the Golgi body.
3. Modification of protein at Golgi body.
4. Separation of secretory vesicle from the Golgi body.
5. Fusion of the vesicles with the cell surface membrane.
6. Contents released/secretion of pores by exocytosis.

This process also works to embed proteins at the cell surface membrane. The difference is the proteins will not be inside the vesicle rather attached to the vesicle.

44
Q

Describe lysosomes

A

They are secretory vesicles.
Very very small
Spherical small sacs.
Function: contain hydrolytic enzymes/lysosozymes,proteases, lipase
Breakdown unwanted structures via hydrolysis in an acidic environments. Worn out or dead cells (REJECT dead organelle) .
In white blood cells lysosozymes digest the bacteria.

45
Q

Describe the mitochondria.

A

Relatively large organelle. (Smaller than chloroplasts and nucleus)
Has double membranes.
Cristae=folded inner membrane
Matrix=Interior solution
Contain 70S ribosomes and small circular DNA.
Divide by binary fission.
Have prokaryotic origin.

46
Q

Describe the function of mitochondria.

A

Site of aerobic respiration.
Synthesize ATP/produce energy in the form of ATP. (REJECT produce energy)
Glucose+Oxygen-> Carbon dioxide+water+energy

47
Q

Describe chloroplasts

A

Relatively large organelle (smaller than nucleus)
Oval shaped
Two membranes
Contains chlorophyll
Thylakoid=Flattened sacs
Grana=Thylakoid stacks.
Stroma=Interior solution
Contain 70s ribosomes, most circular DNA and starch granules.
Divides by binary fission and has prokaryotic origin.

48
Q

What is the function of chlorophyll?

A

Site of photosynthesis.
Two main processes in photosynthesis:
1.Light dependent reaction (aka light reaction).
Light energy is absorbed by water and used to synthesize ATP.
2. Light Independent reaction (aka dark reaction)
ATP used to convert
CO2 into glucose.

49
Q

Describe centrioles and centrosomes.

A

Centrioles are cylindrical.
Made of 9 groups of 3 microtubules.
Not found on plant cells.
Pair of centrioles= centrosome.

50
Q

What is the function of centrioles?

A

Involved in cell division.
Replicates before each cell division and moves to opposite poles. Centrioles are found in pairs at right angles (90°) from each other.
Form centrosomes.
Modified centrioles are found elsewhere for example in flagella/cilia where they act as a microtubule organizing center (MTOC). They organize/assemble microtubules.

51
Q

What is the function of centrosomes?

A

It is a MTOC. Organizes / assembles microtubules for the formation of spindle fibers at opposite poles during cell division.
Aid contraction of spindle fibers to separate sister chromatids.

52
Q

Describe microtubules.

A

Microtubles are very small (~25nm).
They are made from a protein called tubulin. (alpha and beta tubulin).
Dimer’s polymerase to form long protofilaments. 13 proto-filaments is equal to 1 microtubule. Long, rigid hollow, tubes.
Formed and broken down at microtubule organizing centers for example centrosomes, centrioles near flagella/cilia.

53
Q

Describe the function of microtubules.

A

Microtubules make up the cytoskeleton (together with actin filaments). Provides mechanical supports.
Act as an intracellular transport system for movement of vesicles or other components.
They also help in the movement of the cell so as the cytoskeleton changes the cell shape changes creating movement for example the beating of flagella.
They make up spindle fibers and centrioles used in cell division.

54
Q

Describe cilia.

A

Cilia is only found in eukaryotes.
Smaller in diameter than microvilli not to be confused by flagella which is mostly found in prokaryotic cells.
Cilia makes the cell motile (capableof motion) and move rhythmically.
Cilia is a complicated structure made of microtubules.

55
Q

What is the function of cilia?

A

For movement/locomotion. For example ciliated epithelial cells in lungs, paramecium (eukaryotic microbe).

56
Q

Describe the structure of microvilli.

A

Only found in animal cells. Found on epithelial cells in the intestines and kidneys. Finger like extensions of the cell surface membrane.

57
Q

Describe the function of microvilli.

A

Increase surface area of the cell membrane for:
Secretion of enzymes
Absorption
Digestion at the cell surface
Excretion of waste substances.
As they increase the surface area they help increase the rate of diffusion and more movement across the membrane.

58
Q

What happens if we centrifuge cells?

A

Centrifuge is when we rupture cells (remove cell s). The larger structures will sediment first (sink to the bottom).
First the nuclei then the chloroplast then the mitochondria then the plasma membrane and endoplasmic reticulum and lastly the ribosomes.

59
Q

Describe the key features of prokaryotes.

A

Unicellular
Relatively smaller than eukaryotic cells (1-5micrometer)
Simpler in structure as compared to eukaryotes
Divide by binary fission
All bacteria do not have membrane-bound organelles, no nucleus, DNA lies free in cytoplasm in the nucleoid region.

60
Q

What do all bacterias have?

A

All bacteria have a plasma membrane and cytoplasm. Their cell wall is made up of peptidoglycan. Peptidoglycan is a mixture of protein and carbohydrates. The peptidoglycan is made of chains cross-linked by amino acids.Bacteria have 70s ribosomes.
Bacteria have circular DNA.
DNA is naked and is not associated with proteins.

61
Q

Key features are present only in some bacteria?

A

Plasmids which are small circular DNA and code for the non-essential proteins. Several plasmids may be present.

Pillai which is used for sexual reproduction for attachment to other cell surfaces.

Flagellum which is important for locomotion.

Capsule which attaches to different surfaces and is an outer coat for additional protection .

Infolding of the plasma membrane (mesosomes).
Mesomes are usually in bacteria for photosynthesis.

62
Q

What are the common features of what are the common features of prokaryotes mitochondria and chloroplasts.

A

They have similar size between one to five micrometers they all have small circular DNA. They all have 70s ribosomes. They all divide by binary fission.

63
Q

Describe viruses.

A

Non-cellular structure
50 times smaller than bacteria (20~300nm).
It’s much simpler it has no plasma membrane cytoplasm or ribosomes.
It only has:
Nucleic acid core which can be either DNA or RNA.
Capsid which is a protein coat. Some viruses have one coat while others have two.
Some viruses also have an outer envelope made of phospholipids. (Not cell surface membrane).
Some proteins may be present on the outer envelope.

64
Q

Why are viruses considered non-cellular?

A

Viruses are all parasitic and can only reproduce by infecting living cells. Versus uses protein synthesizing machinery of host cells to replicate.

65
Q

Describe how viruses reproduce by infecting living cells

A

Virus binds to cell receptors. Incorporate viral DNA/RNA into cells. Use host cells ribosomes, ER, Golgi to produce viral proteins. Produce more viruse cells.

66
Q

What is an advantage of using a light microscope over other types of microscopes?

A

We can use light microscopes on living cells. This means that we can explore processes such as cell division or movement of cells. Howver sometimes we need to use a stain and these can kill cells.

67
Q

What is a disadvantage of using a light microscope as compared to other microscopes?

A

There can be a problem of resolution which is due to nature of light itself. The maximum resolution of a light microscope is 200nm. If two points are closer than 200nm than they can’t be distinguished as seperate. the resolution of any light microscope will be limited by the wavelength of the light.

68
Q

What is the wavelength of visible light?

A

The wavelength of visible light is around 400nm to 700nm.

69
Q

Define resolution.

A

The ability to distinguish between two objects very close together: the higher the resolution of an image, the greater the detail can be seen.

70
Q

What are the advantages of using an electron microscope over other types of microscopes?

A

Electron microscopes can have a 2000x better resolution than light microscopes.

71
Q

What are the disadvantages of using electron microscopes as compared to other microscopes?

A

The enterior of an electron microscope is a vacuume. We cannot view living specimens.

The electron microscope requires very careful staining of the specimen and the specimen often has to be very thin.

72
Q

What are the two types of electron microscopes?

A

Transmission electron microscopes and scanning electron microscopes.

73
Q

Describe the transmission electron microscope

A

In a transmission electron microscope the electron beam passes through the specimen. The transmission electron micrograph poduces flat 2 dimensional images and only works if the specimen is very thinly sliced. The transmisson electron microscope haa a very high resolution.

74
Q

Describe the scanning electron microscope.

A

In scanning electron microscope the electron beam does not pass through the specimen. Instead electrons are scattered from the surface of the specimen and detected. The scanning electron micrograph produces 3 dimensional images and does not require the specimen to be thinly sliced. Howver the scanning electron microscope has a lower resolution than the transmission electron microscope. It also requires that the specimen is coated with amteal such as gold. This lead to artefacts

75
Q

What is the difference between a transport vesicle and secretory vesicle?

A

Transport vesicles move materials within the cell. Secretory vesicles store and release materials into the cell or to the extracellular environment.