Embryo Flashcards
Spermatogonia and oogonia (gametes) are derived from populations of _ cells
Spermatogonia and oogonia (gametes) are derived from populations of primordial germ cells
Primordial germ cells are produced during week _ of the embryo’s life
Primordial germ cells are produced during week 3 of the embryo’s life
* They initially migrate outside of the embryo into the yolk sac
During week 4, primordial germ cells return to the embryo and follow a path along the gut tube to the _ where the ovary/testis develops
During week 4, primordial germ cells return to the embryo and follow a path along the gut tube to the gonadal ridge where the ovary/testis develops
Primordial germ cells reach the gonadal ridge at about 6 weeks gestation and continue to proliferate via _
Primordial germ cells reach the gonadal ridge at about 6 weeks gestation and continue to proliferate via mitosis
Oogonia multiply by mitosis before beginning meiosis 1 and arresting in _
Oogonia multiply by mitosis before beginning meiosis 1 and arresting in prophase (2N, 4C)
(True/False) Oogonia continue to proliferate and differentiate into primary oocytes
False; by the 7th month of gestation nearly all of the eggs are in the primary oocyte stage and the fetus will stop producing oogonia
* Atresia is the process of cell death of remaining oocytes and some primary oocytes as we age until menopause
What is the sequence of oogenesis?
Primordial germ cell –> oogonia –> primary oocyte –> secondary oocyte –> mature oocyte
Oogenesis occurs during the (follicular/luteal) phase of the ovarian cycle
Oogenesis occurs during the follicular phase of the ovarian cycle –> culminates in ovulation
During the follicular phase, _ is secreted from the pituitary gland and stimulates _ cells surrounding the primary oocyte to enlarge, transform into cuboidal cells and begin secreting estrogens
During the follicular phase, FSH is secreted from the pituitary gland and stimulates granulosa cells surrounding the primary oocyte to enlarge, transform into cuboidal cells and begin secreting estrogens
* About 15-20 primordial follicles in each ovary will begin this but only 1-2 will ovulate
Once activated, primordial follicles transform into primary follicles with the development of _
Once activated, primordial follicles transform into primary follicles with the development of zona pellucida (glycoprotein layer)
Between the granulosa cells and the primary oocyte is the _ layer
Between the granulosa cells and the primary oocyte is the zona pellucida
* Glycoprotein layer that contains receptors and components needed for fertilization
_ cells eventually form the corona radiata
Granulosa cells eventually form the corona radiata
The granulosa cells of the _ will accompany the mature oocyte when it exits the follicle at ovulation
The granulosa cells of the corona radiata will accompany the mature oocyte when it exits the follicle at ovulation
When there is sufficient estrogen in circulation, a surge of _ is released from the pituitary and a _ is released from its meiotic inhibition
When there is sufficient estrogen in circulation, a surge of LH is released from the pituitary and a primary oocyte is released from its meiotic inhibition
This first meiotic division (at ovulation) produces _ + _
This first meiotic division (at ovulation) produces one large secondary oocyte + polar body
The secondary oocyte (formed with ovulation) will be arrested in _
The secondary oocyte (formed with ovulation) will be arrested in metaphase II
The secondary oocyte ruptures through the cells of the mature follicle and gets drawn into the _
The secondary oocyte ruptures through the cells of the mature follicle and gets drawn into the fallopian tube (leaving ovary)
Following ovulation, the ruptured follicle transforms into the _
Following ovulation, the ruptured follicle transforms into the corpus luteum
Unlike the ovary with a finite number of oogonia, the testis maintains a stem cell population of mitotically active _ throughout life
Unlike the ovary with a finite number of oogonia, the testis maintains a stem cell population of mitotically active spermatogonia throughout life
Sequence of spermatogenesis
Spermatogonia –> primary spermatocyte –> secondary spermatocyte –> spermatid –> spermatozoa
Spermatogenesis begins at _
Spermatogenesis begins at puberty
* When spermatogonia start meiosis
Spermatogenesis takes place in the _ and requires supporting cells called _
Spermatogenesis takes place in the seminiferous tubules and requires supporting cells called sertoli cells
The haploid cells (male germ cells) that are produced after meiosis II are called _
The haploid cells (male germ cells) that are produced after meiosis II are called spermatids
The process of transforming spermatids into specialized spermatozoa is called _
The process of transforming spermatids into specialized spermatozoa is called spermiogenesis
Three important features in spermiogenesis that mature the spermatozoa:
Three important features in spermiogenesis that mature the spermatozoa:
1. Acrosome added to head of sperm (enzyme rich)
2. Flagellum powered by mitochondria
3. Pronucleus from condensation of haploid genome
After spermatozoa leave the seminiferous tubules, they enter the _ where they will fully mature
After spermatozoa leave the seminiferous tubules, they enter the epididymus where they will fully mature
During emission, sperm exit the epididymus, travel via the vas deferens to gain access to the urethra; on their way they join secretions from the _ and _
During emission, sperm exit the epididymus, travel via the vas deferens to gain access to the urethra; on their way they join secretions from the seminal vesicles and prostate gland
As the sperm passes through the uterine cavity they undergo _ in which the glycoproteins on the plasma membrane of the sperm head are removed and the acrosome is exposed
As the sperm passes through the uterine cavity they undergo capacitation in which the glycoproteins on the plasma membrane of the sperm head are removed and the acrosome is exposed
(3) steps of fertilization
- Sperm penetrate corona radiata
- Penetration of the zona pellucida; release of contents of the acrosome (acrosomal reaction)
- Fusion of the oocyte and sperm cell membranes
How does the egg respond to the fusion of sperm fushion?
- Cortical reaction: release of cortical granules and lysosomal enzymes that harden the zona pellucida and hydrolyze the sperm receptors; block polyspermy
- Resumption of meiosis II: second asymmetrical cell division occurs
- Egg activation: increase in metabolism in preparation
Fertilization is complete with the first mitotic division of the _
Fertilization is complete with the first mitotic division of the zygote
Fertilization is followed by a series of mitotic divisions inside the hardened _ ; this proliferation is called _ since cytoplasm does not growth and dividing cells get smaller and smaller; these cells are called _
Fertilization is followed by a series of mitotic divisions inside the hardened zona pellucida ; this proliferation is called cleavage since cytoplasm does not growth and dividing cells get smaller and smaller; these cells are called blastomeres
Compaction is the process of packaging the blastomeres into a tightly-packed ball and is mediated by _
Compaction is the process of packaging the blastomeres into a tightly-packed ball and is mediated by E-cadherin
* We get inner and outer cells
Once the embryo consists of 16 cells we call it the _
Once the embryo consists of 16 cells we call it the morula
* Will form the inner cell mass or embryoblast (future embryo proper)
The cells surroundig the morula cells will form the outer cell mass, or _
The cells surroundig the morula cells will form the outer cell mass, or trophoblast
* Trophoblast will contribute to the placenta
Prior to compaction, blastomeres are (totipotent/pluripotent)
Prior to compaction, blastomeres are totipotent
* Capable of developing into all tissues necessary for development (embryonic or placental)
Inner mass cells are (totipotent/pluripotent)
Inner mass cells are pluripotent
Trophoblast cells secrete fluid into the interior of the morula, sequestering the embryoblasts at one pole; the embryo is now called the _
Trophoblast cells secrete fluid into the interior of the morula, sequestering the embryoblasts at one pole; the embryo is now called the blastocyst
Once the embryo reaches the uterine cavity, a molecule on the blastocyst cell membrane lyses a small hole in the zona through which it hatches; this is called _
Once the embryo reaches the uterine cavity, a molecule on the blastocyst cell membrane lyses a small hole in the zona through which it hatches; this is called implantation
Once the embryo adheres to the uterine endometrium, the trophoblast differentiates into the _ (inner layer) and _ (outer layer)
Once the embryo adheres to the uterine endometrium, the trophoblast differentiates into the cytotrophoblast (inner layer) and syncytiotrophoblast (outer layer)
Syncytiotrophoblast provides the mechanism for _ and also begins to secrete _
Syncytiotrophoblast provides the mechanism for endometrial penetration and also begins to secrete beta-hCG
* Invasive tissue; making endometrial penetration possible
* hCG rescues the corpus luteum
Menstrual age of the fetus is usually _ than the fertilization age
Menstrual age of the fetus is usually two weeks greater than the fertilization age
Parturition is usually _ weeks menstrual age and _ weeks fertilization age
Parturition is usually 40 weeks menstrual age and 38 weeks fertilization age
During the second week of development, the embryoblast cells become organized into _
During the second week of development, the embryoblast cells become organized into bilaminar disc
(Hypoblast/Epiblast) is the thin sheet of cells of the bilaminar disc that is adjacent to the blastocyst cavity
Hypoblast is the thin sheet of cells of the bilaminar disc that is adjacent to the blastocyst cavity
(Hypoblast/Epiblast) is the thin sheet of cells of the bilaminar disc that is not in contact with the blastocyst cavity
Epiblast is the thin sheet of cells of the bilaminar disc that is not in contact with the blastocyst cavity
(Hypoblast/Epiblast) is the thin sheet of cells of the bilaminar disc that is ventral
Hypoblast is the thin sheet of cells of the bilaminar disc that is ventral
(Hypoblast/Epiblast) is the thin sheet of cells of the bilaminar disc that is dorsal
Epiblast is the thin sheet of cells of the bilaminar disc that is dorsal
Fluid begins to fill the cavity in the bilaminar disc, called the _
Fluid begins to fill the cavity in the bilaminar disc, called the amnion (amnionic cavity)
The internal, fluid-filled space defined by the hypoblast derived cells is the _
The internal, fluid-filled space defined by the hypoblast derived cells is the primary yolk sac
Cells of the hypoblast also give rise to an additional population of cells, the _
Cells of the hypoblast also give rise to an additional population of cells, the extraembryonic mesoderm
* These will surround primary yolk sac and amnionic cavity
* Eventually cavities get formed and become chorionic cavity
Once the chorionic cavity forms, we get degeneration of the primary yolk sac while the _ becomes the definitive yolk sac of the embryo
Once the chorionic cavity forms, we get degeneration of the primary yolk sac while the secondary yolk sac becomes the definitive yolk sac of the embryo
At the end of week 2 of development, the only attachment between the embryo and the cytotrophoblast is via the _
At the end of week 2 of development, the only attachment between the embryo and the cytotrophoblast is via the connecting stalk
The bilaminar embryonic disc is converted into the trilaminar embryo during week _
The bilaminar embryonic disc is converted into the trilaminar embryo during week 3
What are the three layers of the trilaminar embryo?
- Ectoderm
- Mesoderm
- Endoderm
The central nervous system and the sensory organs are derived from _
The central nervous system and the sensory organs are derived from ectoderm
The epidermis of the skin and its appendages (nails, sweat glands, mammary glands, tooth enamel) is derived from _
The epidermis of the skin and its appendages (nails, sweat glands, mammary glands, tooth enamel) is derived from ectoderm
Neural crest cells are derived from _
Neural crest cells are derived from ectoderm
The neural crest cells give rise to _
The neural crest cells give rise to PNS, melanocytes, endocrine tissues, connective tissues in head and heart
Mesoderm gives rise to mainly _ and _
Mesoderm gives rise to mainly muscle and connective tissues (including blood)
* Therefore most organs come from mesoderm
_ layer derives the epithelial linings of respiratory, gastrointestinal, and urinary tracts
Endoderm layer derives the epithelial linings of respiratory, gastrointestinal, and urinary tracts
The epithelial portions of many glands and organs gets derived from _
The epithelial portions of many glands and organs gets derived from endoderm