Elctron Microscopes

Question 1

What are the two types of electron microscope

Answer 1

Transmission and scanning

Question 2

Which type of electron microscope has a higher resolution

Answer 2

Transmission

Question 3

What is the resolution of transmission microscopes

Answer 3

0.1nm

Question 4

What is the resolution of a scanning microscope

Answer 4

20nm

Question 5

What does a transmission microscope see? 2D or 3D?

Answer 5

Internal structures in 2D

Question 6

What does a scanning microscope see? 2D or 3D?

Answer 6

Only sees external structures in 3D

Question 7

Does the beam of electrons pass through or bounce off of a transmission microscope

Answer 7

Passes through

Question 8

Does the beam of electrons pass through or bounce off a scanning microscope

Answer 8

Bounces off

Question 9

Does the specimen have to be thick or thin in a transmission microscope? Why?

Answer 9

Has to be thin so that electrons can pass through it

Question 10

Does the specimen have to be thick or thin in a scanning microscope? Why?

Answer 10

Doesn’t matter as electrons bounce off of it and don’t need to penetrate the specimen

Question 11

Why must tissues be observed in a vacuum?

Answer 11

So that electrons are not absorbed by air

Question 12

Why must tissues be observed in a vacuum?

Answer 12

So that electrons are not absorbed by air

Question 13

Why is tissues being observed in a vacuum a disadvantage?

Answer 13

You can’t observe living specimens as they have to be dead/die in the vacuum

Question 14

What is an artefact?

Answer 14

Artefacts are things that result from the way that the specimen is prepared

Question 15

Why is an image containing artefacts a disadvantage?

Answer 15

Artefacts may appear on the final finished micrograph but aren’t part of the natural specimen