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CSB327 > ECM in cancer metastasis > Flashcards

ECM in cancer metastasis Flashcards

1
Q

Which cell line’s expression is correlated to MT1 expression?

A

HEY, OVCA429, ES-2

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2
Q

How can zymography be used to see MT1 activity?

A

prommp2 and mmp2 assay

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3
Q

What assay can be used to see if cancer cells migrate with collagen?

A

transwell invasion assay.

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4
Q

What results would western blots show for MT1 expression related to cancer?

A

less pro MT1 and more MT1

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5
Q

why is it hard to narrow down MT1-MMP to be the only effector of cancer?

A

no specific inhibitor.

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6
Q

How can we narrow down MT1-MMP to be the only

A

dont know

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7
Q

What are some physical characteristics of collagen in tumor environment?

A

TACS-1: dense around initial tumor
TACS-2: taught. straight collagen around non invading large tumors
TACS-3: radial arrangement around invading tumors

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8
Q

Why are invading tumors with radially arranged collagen fibres?

A

its their highways

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9
Q

Do cancer cells like rigid dense matrices?

A

hell ya. easier to move around

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10
Q

Do cancer cells like rigid dense matrices?

A

hell ya. easier to move around

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11
Q

What can decrease cancer movement by lowering rigidity?

A

Lox inhibitors

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12
Q

Peritoneal metastasis is inhibited by what?

A

Mesothelial cells

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13
Q

Peritoneal metastasis is enhanced by what?

A

TNF-a

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14
Q

Is MT1-MMP inhibitor a good idea for cancer treatment?

A

No. First there is not specific inhibitor i.e. TIMP2 would be inhibited soluble MMPs and GM6001 would inhibit all MMPs.
Also MMPs are required in other functions such as wound healing, angiogenesis

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15
Q

Why is spheroids cancer cells bad?

A

resistant to chemotherapeutics

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16
Q

What breaks down the mesothelial layer?

A

TNF-a

17
Q

What is the purpose of mesothelial layer in omentum?

A

conceal the collagen fibrils in peritoneal matrix

18
Q

Why is LOX inhibtor good against cancer?

A

LOX catalyzes the conversion of lysine molecules into highly reactive aldehydes that form cross-links in extracellular matrix proteins.

19
Q

What one good and three bad cancer treatment for peritoneal metastasis?

A

good: LOX inhibitor
bad: TIMP1, TIMP2, TNF-a inhibitor

20
Q

Why is TNF-a inhibitor a bad idea?

A

multiple side effects

  • lymphoma,
  • infections
  • demyelinating disease
  • production of auto-antibodies,
  • congestive heart failure
  • injection site reactions
21
Q

How does cancer cells initially start passing through in the mesothelial boundary?

A

milky spots.

rounded, gapped mesothelial cells due to TNF-a

22
Q

Why is milky spot white?

A

macrophages

23
Q

How does MT1 promote cancer?

A

focalized pericellular proteolysis

CSB327 (5 decks)

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