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CSB327 > BSP & osteoponton > Flashcards

BSP & osteoponton Flashcards

1
Q

What are two types of bones?

A

Hard cortical bone

spongy cancellous bone

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2
Q

Cancellous bone composition

A

25% organic

  • 90% collagen I and V
  • 10% other stuff

70% hydroxyapatite
5% water

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3
Q

what is ceramic?

A

an inorganic compound that is heated and cooled quickly

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4
Q

what is bioceramic? example?

A

a biocompatible ceramic

calcium hydroxyapatite

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5
Q

Do osteoblasts and osteoclasts come from the same progenitor stem cells?

A

no.
osteoblasts < mesenchymal SC
osteoclasts < hematopoietic SC

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6
Q

What do Osteoblasts become after mineralization?

A

osteocytes

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7
Q

(commitment) Mesenchymal progenitor > osteo-progenitor. Which signals?

A

BMP, runx, wnt

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8
Q

(proliferation) Osteo-progenitor > Immature osteoblast. Which signals?

A

runx, osterix

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9
Q

(mature matrix) immature osteoblats > osteoblats. Which signals?

A

BSP, alkaline phosphatase, collagen I

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10
Q

What is the matrix produced around osteoblasts called and what happens to it

A

osteoid and it gets mineralized

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11
Q

What fits in the gaps of the spongy bones?

A

bone marrow

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12
Q

What is the formula for hydroxyapatite

A

Ca10 (PO4)6 (OH)2

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13
Q

What are two of the important non-collagenous proteins that bind to hydroxyapatite?

A

BSP (bone sialoprotein)

OPN (Osteopontin)

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14
Q

What are some characterists similar and different between BSP and OPN?

A

similarities

  • binds to hydroxyapatite
  • binds to RGD integrins
  • highly phosphorylated

Differences

  • BSP: 2-3 glutamic motif
  • BSP is more tissue specific
  • BSP is highly sulfated too
  • BSP has high sialic acid content
  • OPN: polyaspartic motif
  • OPN is broad tissue specific
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15
Q

What are some characterists similar and different between BSP and OPN?

A

similarities

  • binds to hydroxyapatite
  • binds to RGD integrins
  • highly phosphorylated

Differences

  • BSP: 2-3 glutamic motif
  • BSP is more tissue specific
  • BSP is highly sulfated too
  • BSP has high sialic acid content
  • OPN: polyaspartic motif
  • OPN is broad tissue specific
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16
Q

What are some characterists similar and different between BSP and OPN?

A

similarities

  • binds to hydroxyapatite
  • binds to RGD integrins
  • highly phosphorylated

Differences

  • BSP: 2-3 glutamic motif
  • BSP is more tissue specific
  • BSP is highly sulfated too
  • BSP has high sialic acid content
  • OPN: polyaspartic motif
  • OPN is broad tissue specific
17
Q

Which proteins are expressed during the differentiation phase?

A

Alkaline Phosphatase

BSP (also early mineralization)

18
Q

Which proteins are expressed during the Mineralization phase?

A

OPN

SPARC (always)

19
Q

Draw a western blot for termporal expression of all protein

20
Q

Where are early crystals formed?
What are these crystals made of?
What promotes this growth?

A

in the collagen hole zones

hydroxyapatite crystals

BSP
vesicles (w/ alkaline phosphatase and nucleotide
pyrophosphatase/phosphodiesterase, Annexin)

21
Q

What are the main places the vesicles start minerlizing?

A

osteoid
cartiliage
dentin

22
Q

What brings in calcium in the vesicles?

23
Q

What brings in phosphate in the vesicles?

A

Type III Na dependent transporters

24
Q

What do MMPs do inside vesicles?

A

growth factor activation

25
Q

Which molecule facilitates the transport of crystals to the collagen?

A

annexin transporters bind Ca2+ and collagen and does this

26
Q

KO of which gene would cause no osteoblast differntiation in the endochondral and intramembranous skeleton?

27
Q

In Runx/Cbfa-1 null mice, osteoblast differentiation

Is arrested in?

A

endochondral and intramembranous skeleton

28
Q

osterix contains which motif?

A

zinc finger

29
Q

without which transcription factor, no mineralization is shown?

30
Q

What reabsorbs bone? What is its precursor?

A

osteoclasts.

myeloid progenitor

31
Q

What is needed for osteoclasts to form instead of macrophages?

A

RANKL on osteoblasts

32
Q

What happens to osteoclasts after their work?

33
Q

How do osteoclasts bind to bone?

A

OPN and integrin avb3

34
Q

How is the mineral dissolved?

A

By forming a ruffled ring with actin where proton is pimped lower ph. dissolving the mineral in lacuna.

35
Q

Matrix-GLA-protein (MGP) KO characteristic?

What else shows this result?

How to know if the effects are actually from MGP

A

mineralization of the arteries

OPN -/-

bring back KO gene

36
Q

How to stain for phosphate?

A

Van Kossa stain

photochemical reaction in which silver ions react with phosphate (not calcium)
in the Presence of acidic materials.

37
Q

How to stain for calcium?

A

alizarin red

CSB327 (5 decks)

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