Drawing Gluconeogenesis Flashcards
What is the first step in gluconeogenesis and draw it?
(2) pyruvate -> (2) oxaloacetate
2ATP -> 2ADP
What is the enzyme that catalyzes pyruvate to oxaloacetate?
Pyruvate carboxylase
Draw oxaloacetate
(-)O-C(=O)-C(=O)-CH2-C(=O)-O(-)
What is the second step in gluconeogenesis and draw it
(2) oxaloacetate -> (2) phosphoenolpyruvate
2GTP -> 2GDP
What is the enzyme for oxaloacetate to phosphoenolpyruvate?
PEP carboxykinase
What is step 3 of gluconeogenesis and draw it
(2) Phosphoenolpyruvate < > (2) 2-phosphoglycerate
What is the enzyme for phosphoenolpyruvate to 2-phosphoglycerate?
Enolase
What is step 4 of gluconeogenesis and draw it
(2) 2-phosphoglycerate < > (2) 3-phosphoglycerate
What is the enzyme for 2-phosphoglycerate to 3-phosphoglycerate?
Phosphoglycero-mutase
What is step 5 of gluconeogenesis and draw it
(2) 3-phosphoglycerate < > (2) 1,3-bisphosphoglycerate
2ATP -> 2ADP
What is the enzyme for 3-phosphoglycerate to 1,3-bisphosphoglycerate?
Phosphoglycerate kinase
What is step 6 of gluconeogenesis and draw it
(2) 1,3-bisphosphoglycerate < > (2) glyceraldehyde 3-phosphate
2NADH + H+ -> 2NAD+ + 2Pi
What enzyme catalyzes 1,3-bisphosphoglycerate to glyceraldehyde 3-phosphate?
Glyceraldehyde 3-phosphate dehydrogenase
What is step 7 of gluconeogenesis and draw it
(2) Glyceraldehyde 3-phosphate < > (2) Dihydroxyacetone phosphate
What enzyme catalyzes glyceraldehyde 3-phosphate to dihydroxyacetone phosphate?
Triose phosphate isomerase
What is step 8 of gluconeogenesis and draw it
(2) Glyceraldehyde 3-phosphate < > Fructose 1,6-bisphosphate
What enzyme catalyzes glyceraldehyde 3-phosphate to fructose 1,6-bisphosphate
Aldolase
What is step 9 of gluconeogenesis and draw it
Fructose 1,6-bisphosphate -> fructose 6-phosphate
H2O -> Pi
What enzyme catalyzes fructose 1,6-bisphosphate to fructose 6-phosphate?
Phosphofructo-kinase-1
What is step 10 of gluconeogenesis and draw it
Fructose 6-phosphate < > glucose 6-phosphate
What enzyme catalyzes fructose 6-phosphate to glucose 6-phosphate?
Fructose 1,6-bisphophosphatase
What is step 11 of gluconeogenesis and draw it
Glucose 6-phosphate -> glucose
H2O -> Pi
What enzyme catalyzes glucose 6-phosphate to glucose?
Glucose 6-phosphatase
Draw formation of oxaloacetate in mitochondria matrix to cytoplasm from pyruvate
Pyruvate -> Oxaloacetate -> Malate —–> Malate -> Oxaloacetate
1st part: CO2 + ATP -> ADP + Pi
2nd part: NADH + H+ -> NAD+
malate back to oxaloacetate now in cytoplasm rxn: NAD+ -> NADH + H+
