Drawing Citric Acid Cycle

Question 1

Enzyme for Pyruvate to Acetyl CoA and products of that reaction?

Answer 1

Pyruvate dehydrogenase

Question 2

What is added/released from reaction of Pyruvate to Acetyl CoA?

Answer 2

NAD+ + CoA-SH -> NADH + H+ + CO2

Question 3

Draw Acetyl CoA

Answer 3

CH3-C(=O)-S-CoA

Question 4

Enzyme for Acetyl CoA + Oxaloacetate to Citrate?

Answer 4

Citrate Synthase

Question 5

What is added/released from the reaction of Acetyl CoA + Oxaloacatate to Citrate?

Answer 5

H2O -> CoA-SH

Question 6

Draw Citrate

Answer 6

(-)OOC-CH2-C(-OH and COO-)-CH2-COO(-)

Question 7

Enzyme for Citrate to Isocitrate?

Answer 7

Aconitase

Question 8

Draw Isocitrate

Answer 8

(-)OOC-CH(OH)-CH(COO-)-CH2-COO(-)

Question 9

Enzyme for isocitrate to alpha-ketoglutarate?

Answer 9

Isocitrate dehydrogenase

Question 10

What is added/released from Isocitrate to alpha-ketoglutarate?

Answer 10

NAD+ -> NADH + CO2

Question 11

Draw alpha-ketoglutarate

Answer 11

(-)OOC-C(=O)-CH2-CH2-COO(-)

Question 12

Enzyme for alpha-ketoglutarate to succinyl CoA?

Answer 12

alpha-ketoglutarate dehydrogenase complex

Question 13

What is added/released from alpha-ketoglutarate to succinyl CoA?

Answer 13

NAD+ + CoA -> NADH + CO2

Question 14

Draw Succinyl CoA

Answer 14

CoA-S-C(=O)-CH2-CH2-COO(-)

Question 15

Enzyme for succinyl CoA to Succinate?

Answer 15

Succinyl CoA synthetase

Question 16

What is added/released from Succinyl CoA to Succinate?

Answer 16

ADP + Pi -> ATP + CoA

Question 17

Draw Succinate

Answer 17

(-)OOC-CH2-CH2-COO(-)

Question 18

Enzyme for succinate to fumarate?

Answer 18

Succinate dehydrogenase

Question 19

What is added/released from Succinate to Fumrate?

Answer 19

FAD -> FADH2

Question 20

Draw Fumarate

Answer 20

(-)OOC-CH=CH-COO(-)

Question 21

Enzyme for fumarate to malate?

Answer 21

Fumarase

Question 22

What is added to fumarate to malate reaction?

Answer 22

H2O

Question 23

Draw Malate

Answer 23

(-)OOC-CH(OH)-CH2-COO(-)

Question 24

Enzyme for malate to oxaloacetate?

Answer 24

Malate dehydrogenase

Question 25

What is added/released from malate to oxaloacetate?

Answer 25

NAD+ -> NADH + H+

Question 26

Draw Oxaloacetate

Answer 26

(-)OOC-C(=O)-CH2-COO(-)

Question 27

Total number of NADH and FADH2 from 1 molecule of pyruvate?

Answer 27

3 NADH 1 FADH2

Question 28

Total number of ATP made from CAC?

Answer 28

10
1 ATP released
NADH -> 2.5ATP x 3 = 7.5 ATP
FADH2 -> 1.5ATP x 1 = 1.5 ATP

Question 29

Where does the CAC occur?

Answer 29

Mitochondria matrix