(DON'T NEED)Biotechnology

Question 1

How is genetic engineering used to make insulin?

Answer 1

• The human gene responsible for making insulin is cut out using an enzyme called restriction enzyme.
• The plasmid of a bacteria is also cut out using restriction enzymes.
• The human insulin gene is placed into the plasmid
• An enzyme called ligase joins the ends of the insulin gene to the cut ends of the plasmid.
• The plasmid is now called recombinant plasmid or recominant DNA.
• The recombinant DNA is placed into the bacteria and grown to create insulin.