DNA Test Flashcards

1
Q

Define triplet

A

3 bases on DNA that code for 1 amino acid

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2
Q

Define codon

A

3 bases on mRNA that code for 1 amino acid

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3
Q

Define anticode

A

3 bases on tRNA that code for 1 amino acid

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4
Q

Define mutation

A

Any change in a DNA code

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5
Q

Know the three types of DNA mutations

A

Substitutions, insertion, and deletion

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6
Q

What is substitution?

A

a change in 1 letter in an amino acid sequence

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7
Q

What is insertion?

A

Adding an extra letter in an amino acid sequence

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8
Q

What is deletion?

A

Removing a letter from an amino acid sequence

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9
Q

Know the possible outcomes of DNA mutations

A

Missense, silent, premature stop, and frame shift

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10
Q

What is missense?

A

A change in one amino acid

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11
Q

What is silent?

A

Amino acid not changed

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12
Q

What is premature stop?

A

Chain ends early

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13
Q

What is frame shift?

A
  • Changes all amino acids past that point of mutation
  • Caused by either an insertion or deletion
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14
Q

Define transcription

A

Going from DNA and making mRNA.

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15
Q

Where does transcription take place?

A

The Nucleus

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16
Q

What are the steps of transcription?

A

Initiation (RNA polymerase binds to the promoter region on DNA), elongation (RNA polymerase adds complementary RNA nucleotides to the growing RNA chain), and termination (RNA polymerase reaches a termination sequence and releases the completed RNA transcript).

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17
Q

What enzyme “unzips” and builds the RNA molecule?

A

RNA polymerase

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18
Q

Define translation

A

mRNA –> protein

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19
Q

Where does translation take place?

A

The Ribosomes

20
Q

What are the steps of translation?

A

mRNA binds to the ribosome, tRNA brings amino acids to the ribosome, and tRNA puts its amino acid according to the code of the mRNA.

21
Q

What are proteins made of?

A

Many amino acids bonded together.

22
Q

What determines the type of protein?

A

The order of amino acids.

23
Q

What does DNA stand for?

A

Deoxyribonucleic Acid

24
Q

What is the function of DNA?

A

Blueprint/code for making proteins

25
DNA is a polymer. What is a polymer?
A molecule made of many repeating units
26
What is the monomer of DNA called?
Nucleotide
27
What three parts make up a nucleotide?
Sugar, phosphate, and a nitrogenis base
28
What are the four nitrogen bases?
Adenine, guanine, thyanine, and cytosine
29
Is DNA double or single stranded?
Double stranded
30
What does the structure of DNA look like?
A twisted ladder (double helix)
31
What are the sides of the ladder made of?
Phosphate and sugar
32
What are the rungs of a ladder made of?
Base pairs
33
The sugar-phosphate chain is connected with what?
Covalent bonds
34
The paired nitrogen bases are held together by what?
Hydrogen bonds
35
What does C pair with?
G (three bonds)
36
What does A pair with?
T (two bonds)
37
Is DNA anti-parallel?
Yes
38
Know how to write amino acid sequences DNA non template strand: 5'ATGC
-DNA template strand: 3'TACG -mRNA strand: 5'AUGC -tRNA anticodons: 3'UACG
39
What are the steps of DNA replication?
40
What enzyme "unzips" the DNA molecule?
Helicase
41
What enzyme builds the new DNA strands?
DNA polymerase
42
After replication what does each DNA molecule consist of?
An old strand and a new strand
43
What does RNA stand for?
Ribonucleic Acid
44
Know the three differences between DNA and RNA
1) DNA is double stranded and RNA is single stranded 2) RNA has the sugar ribose, DNA has deoxyribose 3) RNA has uracil (U) instead of T
45
What are the functions of mRNA and tRNA?
tRNA transfers amino acids and mRNA gets the code on DNA to the ribosomes in the cytoplasm