DNA Technology

Question 1

Restriction Enzymes

Answer 1

Bacterial enzymes used to cut DNA molecules at specific recognition sequences.

Question 2

Cloning in a plasmid vector

Answer 2

Method used to isolate and amplify in bacteria a segment of a specific gene of interest via plasmid

Question 3

Cloning in a Cosmid vector

Answer 3

used to isolate and amplify in bacteria a segment of a specific gene of interest by utilizing the COS packaging sequences of bacteriophage lambda to package recombinant DNA into phage particles.

Question 4

Cloning in a Yeast Artificial Chromosome (YAC)

Answer 4

Method used to isolate and carry in yeast (Saccharomyces cerevisiae) a segment of a human chromosome.

Question 5

Cloning in a Bacterial Artificial Chromosome (BAC)

Answer 5

Method used to isolate and carry in bacteria a segment of a human chromosome.

Question 6

Genomic and cDNA Libraries

Answer 6

Method used to isolate and carry in a cloning vector a large collection of clones collectively covering the entire genome of an organism of interest