DNA Technology Flashcards
Sticky ends
Staggered single stranded end piece extending from out DNA
Restriction Enzyme
Enzymes that out dna of specific sequences
Recombinant DNA
DNA formed by joining pieces from different sources
Chromatography
Collective term for a set of laboratory technique for the seperation of mixtures
Gel Electrophoresis
Technique which uses electrical current to separate molecules
Agarose
The gel used, and acts as a filter
DNA fingerprinting
Using electrophoresis and enzymes to identify aspects of DNA
Probe
Single stranded radioactively labeled nucleic acid segments, that are used to find genes
PCR
A technique that makes many copies of a certain segment of DNA without using living cells
Human Genome Project
International project to find all gene codes in a human
James Watson
First head of HGP
Francis Collins
Final head of HGP
Gene sequencing
Using gel electrophoresis to find the gene code
Genetic engineering
The alteration of DNA through uses of enzymes and vectors
Vector
A substance used to insert a gene into a new cell
Plasmid
Circular DNA of lipids
Liposomes
Balls of lipids
Virus
Naturally insert their DNA when they infect their host
Transgenic
An organism containing a foreign gene
Also called GMO=genetically modified organism
Genetic Engineering
The alteration of DNA through uses of enzymes and vectors
Gene therapy
The insertion of genes into human to correct illnesses
Ashanti DeSilva
Missing enzymes so has no immune system (–immune defficency)
- added a gene to her white blood cells
Southern blot
Uses probes and x ray film to make a DNA fingerprint permanent
PGD
Diagnosis studies an embryo created through IVF’s gene before implanting it in a uterus
