DNA Technology Flashcards

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0
Q

Sticky ends

A

Staggered single stranded end piece extending from out DNA

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1
Q

Restriction Enzyme

A

Enzymes that out dna of specific sequences

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2
Q

Recombinant DNA

A

DNA formed by joining pieces from different sources

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3
Q

Chromatography

A

Collective term for a set of laboratory technique for the seperation of mixtures

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4
Q

Gel Electrophoresis

A

Technique which uses electrical current to separate molecules

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5
Q

Agarose

A

The gel used, and acts as a filter

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6
Q

DNA fingerprinting

A

Using electrophoresis and enzymes to identify aspects of DNA

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7
Q

Probe

A

Single stranded radioactively labeled nucleic acid segments, that are used to find genes

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8
Q

PCR

A

A technique that makes many copies of a certain segment of DNA without using living cells

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9
Q

Human Genome Project

A

International project to find all gene codes in a human

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10
Q

James Watson

A

First head of HGP

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11
Q

Francis Collins

A

Final head of HGP

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12
Q

Gene sequencing

A

Using gel electrophoresis to find the gene code

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13
Q

Genetic engineering

A

The alteration of DNA through uses of enzymes and vectors

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14
Q

Vector

A

A substance used to insert a gene into a new cell

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15
Q

Plasmid

A

Circular DNA of lipids

16
Q

Liposomes

A

Balls of lipids

17
Q

Virus

A

Naturally insert their DNA when they infect their host

18
Q

Transgenic

A

An organism containing a foreign gene

Also called GMO=genetically modified organism

19
Q

Genetic Engineering

A

The alteration of DNA through uses of enzymes and vectors

20
Q

Gene therapy

A

The insertion of genes into human to correct illnesses

21
Q

Ashanti DeSilva

A

Missing enzymes so has no immune system (–immune defficency)
- added a gene to her white blood cells

22
Q

Southern blot

A

Uses probes and x ray film to make a DNA fingerprint permanent

23
Q

PGD

A

Diagnosis studies an embryo created through IVF’s gene before implanting it in a uterus

24
Q

IVF

A

The science name for a “test tube” baby

25
Q

Therapeutic cloning

A

Created by inserting your nucleus into an egg

26
Q

Multipotent

A

Cells that are partially specialized

Found in your body’s bone marrow; found in a baby’s umbilical cord

27
Q

Pluripotent

A

Embryonic version that are less specialized