DNA sequencing Flashcards
DNA sequencing 1/11
DNA is mixed with primers, polymerase, nucleotide and terminators
DNA sequencing 2/11
Placed in a thermal cycler
DNA sequencing 3/11
at 96oc the DNA strands seperate
DNA sequencing 4/11
at 50oc primers anneal to strand ends
DNA sequencing 5/11
at 60oc DNA polyemrase starts to build new DNA strands
DNA sequencing 6/11
Everytime a terminator is used instead of a nucleotide no more bases can be added
DNA sequencing 7/11
This causes many bases varying in length
DNA sequencing 8/11
DNA fragments are separated by capillary sequencing
DNA sequencing 9/11
Florescent tags used to identify terminator bases using lasers
DNA sequencing 10/11
Order of nucleotides shows sequence of new complimentary strands
DNA sequencing 11/11
Computer anaylses overlaps in code in fragments