DNA sequencing Flashcards

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1
Q

DNA sequencing 1/11

A

DNA is mixed with primers, polymerase, nucleotide and terminators

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2
Q

DNA sequencing 2/11

A

Placed in a thermal cycler

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3
Q

DNA sequencing 3/11

A

at 96oc the DNA strands seperate

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4
Q

DNA sequencing 4/11

A

at 50oc primers anneal to strand ends

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5
Q

DNA sequencing 5/11

A

at 60oc DNA polyemrase starts to build new DNA strands

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6
Q

DNA sequencing 6/11

A

Everytime a terminator is used instead of a nucleotide no more bases can be added

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7
Q

DNA sequencing 7/11

A

This causes many bases varying in length

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8
Q

DNA sequencing 8/11

A

DNA fragments are separated by capillary sequencing

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9
Q

DNA sequencing 9/11

A

Florescent tags used to identify terminator bases using lasers

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10
Q

DNA sequencing 10/11

A

Order of nucleotides shows sequence of new complimentary strands

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11
Q

DNA sequencing 11/11

A

Computer anaylses overlaps in code in fragments

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