DNA replication and protein synthesis Flashcards
What does DNA Helicase do ?
Splits the dna strand into two single strands .
What happens after the dna strand is split in two ?
Free floating nucleotides go towards their complementary bases and attach onto them.
What does DNA polymerase do ?
Catalyzes the reactions between the sugar phosphate backbones of the nucleotides .
What is the leading strand ?
It runs three prime to five prime so the dna polymerase can run allong it straight after the dna helicase therefore it can catalyze the reactions quickly.
What is the lagging strand ?
It runs five prime to three rime therefore the dna polymerase cant run from the end of the strand straight away so has to wait till it has unzipped slightly then put it into fragments , meaning it takes more time to add nucleotides on this strand .
What is an okazaki fragment?
fragments of the strand needs to split off so that the dna polymerase can catalyze the reaction of joining the sugar phosphate backbones .
what does dna ligase do ?
It is the enzyme that puts the okazaki fragments back together to form the complete dna strand.
What is meant by semi-conservative ?
Each dna strand has half of the origional one
What id the promoter base ?
It is the base that is on a split up dna strand that tells the ribose nucleotides where to start attaching on for that gene .
What is the antisense strand ?
the strand of dna that the rna nucleotides attach onto using complementary base pairing .
What is the sense strand ?
The strand that is copied, as the new pre mrna strand has used complementary base pairing to match up with the other strand .
Whta does rna polymerase do ?
It catalyzes the reactions between the the nucleotides and forms strong covalent phosphodiester bonds.
After how many base pairs does the mrna molecule peel off from the dna strand ?
8
What are introns ?
the non coding bases in a sequence that are removed through splicing before becoming mrna.
What are exons ?
the coding bases in a sequence that are the bases needed for that gene therefore they stay .