DNA replication Flashcards
what specific part of the DNA does DNA replication start
origin of replication
the origin of replication denotes the are of active replication known as
replication fork
what enzyme separates the strands of double helix
helicase
what stabilize the newly single stranded regions
single stranded binding proteins
This enzyme makes sure that the double stranded dna outside the replication fork do not supercoil
DNA gyrase
What enzyme catalyzes the addition of new nucelotides to the newly single stranded regions
DNA polymerase
what proteins help hols the DNA polymerase in place
Beta clamp
Clamp loader
Short sequence of DNA like primers have to be paired to the template strands to start the replication by what enzyme
Primase
Continuous synthesis
3’-5’ leading strand
discontinuous synthesis
5’-3’ lagging strand
lagging strand makes
okazaki fragments
what enzyme adds short sequences of nucleotides, okazaki fragments, to the primer filling in the gap
DNA polymerase III
enzyme that replaces primase with DNA nucleotides
DNA polymerase 1
enzymes that ensures that the bonds in between the fragments and the nuceoltides are glued together
DNA ligase
Can DNA polymerase begin to add a nucleotide without a primer?
NO