DNA replication Flashcards
DNA in prokaryotic cells
no nucleus, circular chromosomes located in a region of cytoplasm called the nucleoid, does not have histones
DNA in eukaryotic cells
linear chromosomes found in the nucleus, in the form of chromatin and is coiled around proteins called histones
DNA replication
occurs in the nucleus of eukaryotic cells and in the cytoplasm of prokaryotic cells
evolution of cells
evidence that prokaryotic cells came before eukaryotic, as DNA found in chloroplast and mitochondria have circular chromosomes
semi-conservative
DNA replication is described as this as the new DNA molecules both consist of one old and one new strand
stage 1 of DNA replication
- enzyme helicase breaks the weak hydrogen bonds between the bases
stage 2 of DNA replication
- free DNA nucleotides are attracted to the exposed complementary bases on the template strands. hydrogen bonds form between these bases
stage 3 of DNA replication
the enzyme DNA polymerase joins the sugar and phosphate groups of adjacent nucleotides creating the sugar-phosphate backbone of the newly synthesized strand
stage 4 of DNA replication
two new DNA molecules are formed
leading and lagging strands
the leading strand runs in the 5’ to 3’ direction, the lagging strand runs in the 3’ to 5’ direction. DNA polymerase builds the new strand in the 3’ to 5’ direction, therefore the