DNA Replication Flashcards

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1
Q

What is the central dogma?

A

Genetic information can be passed from nucleic acid to nucleic acid or from nucleic acid to protein. Not from protein to nucleic acid

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2
Q

What is gene expression?

A

The production of a functional protein

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3
Q

What are DNA and RNA?

A

Nucleotides

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4
Q

What is at 5’ on DNA?

A

Terminal phosphate group

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5
Q

What is at 3’ on DNA?

A

Terminal hydroxyl group

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6
Q

How do 2 strands of DNA run?

A

Anti-parallel

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7
Q

When does DNA replication take place?

A

Interphase of mitosis and meoisis

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8
Q

What does semi-conservative mean?

A

Each parent strand is a template for a new strand and the 2 new DNA molecules have 1 old and 1 new strand

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9
Q

How is DNA unwound?

A

Helicases

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10
Q

How do DNA strands grow?

A

From 5’ end to 3’ end

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11
Q

How is the leading strand synthesised?

A

By the DNA polymerase enzyme

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12
Q

How is the lagging strand synthesised?

A

In chunks called Okazaki fragments which are joined by ligase enzymes

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13
Q

What is transcription?

A

Copying the information from DNA into a mRNA

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14
Q

Why is RNA less stable than DNA?

A

It has an extra -OH group

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15
Q

What are the 3 phases of transcription?

A

Initiation (unwinding), Elongation (transcribing) and Termination ( finished product)

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16
Q

How is transcription carried out?

A

Using RNA polymerase

17
Q

What is mRNA?

A

messengerRNA used to carry genetic code from DNA in nucleus to ribosomes. It is roughly 1000 nucleotides long

18
Q

What is rRNA

A

ribosomalRNA is a main component of ribosomes and is responsible for linking amino acids together in the correct order

19
Q

What is tRNA?

A

tRNA bring amino acids to the ribosomes so that a protein can be assembled. It is roughly 80 nucleotides long