DNA Replication Flashcards
What is the central dogma?
Genetic information can be passed from nucleic acid to nucleic acid or from nucleic acid to protein. Not from protein to nucleic acid
What is gene expression?
The production of a functional protein
What are DNA and RNA?
Nucleotides
What is at 5’ on DNA?
Terminal phosphate group
What is at 3’ on DNA?
Terminal hydroxyl group
How do 2 strands of DNA run?
Anti-parallel
When does DNA replication take place?
Interphase of mitosis and meoisis
What does semi-conservative mean?
Each parent strand is a template for a new strand and the 2 new DNA molecules have 1 old and 1 new strand
How is DNA unwound?
Helicases
How do DNA strands grow?
From 5’ end to 3’ end
How is the leading strand synthesised?
By the DNA polymerase enzyme
How is the lagging strand synthesised?
In chunks called Okazaki fragments which are joined by ligase enzymes
What is transcription?
Copying the information from DNA into a mRNA
Why is RNA less stable than DNA?
It has an extra -OH group
What are the 3 phases of transcription?
Initiation (unwinding), Elongation (transcribing) and Termination ( finished product)