DNA Replication Flashcards
Guanine base pairing
Only with cytosine
3 hydrogen bonds
Adenine base pairing
Only with Thymine
2 hydrogen bonds
Conservative model
Parent strand reforms after replication
Dispersive model
New strand contains old and new fragments
Semi-conservative model
Each newly synthesized helix contains one new and one old strand
Correct model
Replication bubble
Proteins attach to DNA and separate strands
Nucleoside triphosphate
Energy for DNA polymerase
Sugar and nitrogenous base plus 3 phosphate groups
2 phosphates get clipped off- undergo hydrolysis to create energy
DNA polymerase III
Adds nucleotides to free 3’ end of DNA strand
Okazaki fragments
Sections of newly synthesized DNA
Formed on lagging strand
DNA ligase
Forms bonds between Okazaki fragments, joining them together
Primer
Short strand of RNA that DNA pol III adds nucleotides to
Primase
Joins RNA nucleotides to make primer that is complementary to DNA strand
DNA polymerase I
Replaces RNA primer with primers of DNA nucleotides
Helicase
Enzymes that untwists DNA helix @ replication fork
Topoisomerase
Relieves strain caused by helicase as it unwinds DNA