DNA recombinant

Question 1

Applications of recombinant technology

Answer 1

1) Quantitative Preparation of Bio-molecules
❖ If molecules are isolated from higher organisms,
the availability will be greatly limited, eg. GH
❖ By means of recombinant technology, large scale
availability is now assured.

2) Risk of Contamination (污染) is Eliminated
❖ to make sure that the preparations of vaccines or
clotting factors are free from contaminants eg.
hepatitis B particles.

Question 2

What is the pre-requisite and function of blunt end

Answer 2

FX: Facilitate a straightforward and close connection of DNA withoutany extra embellishment or extensions

Pre-requisite: resultant DNA cut with a flat, flush end

Question 3

What is the pre-requisite and function of sticky end

Answer 3

FX: Allow for recombinent DNA to be made by cutting and pasting molecules of DNA together

Pre-requisite: resultant DNA cut in uneven way, resulting extentions on both strands

Question 4

What is the pre-requisite and function of Restriction endonucleases

Answer 4

FX: act as a molecular scissors, recognize specific sequences in DNA and cut the DNA

Pre-requisite: split the gene from parent DNA to transfer gene