DNA and Protein Synthesis Flashcards

1
Q

Dna replicatio has 3 steps

A
  1. initiation
  2. elongation
  3. termination
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2
Q

what is ORC

A

Origin recognition complex - similar to Ecoli
recognizes the origin
replication element on the replication sequences

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3
Q

Helicase

A

facilitates the unwinding of the double stranded DNA to single. replication fork

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4
Q

What is MCM

A

Mini chromosome maintenance complex

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5
Q

Is MCM a type of Helicase. Yes or No

A

Yes, it is a type of helicase

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6
Q

What is RPA

A

Replication protein A- Single stranded binding protein

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7
Q

what is reanneal

A

To convert (nucleic acid) from a single-stranded to a double-stranded form, especially after denaturation.

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8
Q

What is the function of RPA

A

prevents the strand of the dna to reaneal

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9
Q

an enzyme that adds a stretch of RNA molecules that assits in polymerazation

A

Primase

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10
Q

DNA polymerase

A

replicative enzyme DNA dependent/ uses dna as template to make double stranded dna molecule

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11
Q

Does DNA polymarase check if correct base parining is occuring

A

YEs

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12
Q

what is the main replicative enzyme

A

DNA polymerase Delta

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13
Q

Ligase

A

enzyme that forms 2 DNA fragmets by catalyzing the formation of ester bonds

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14
Q

Enzymes and Proteins for RNA production

A

RNA Polymerase

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15
Q

is tha carrier of the genetic code

A

MRNA

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16
Q

is a collection of codons

A

Genetic code

17
Q

this is the Start codon

18
Q

for 20 AA there should be

19
Q

how many nucleotides are there in 1 codon

20
Q

Usually stop codons

21
Q

TRNA is sometimes called

A

adapter molecule

22
Q

2 important site of Trna

A

Amino acid attachment

anti codon

23
Q

convertion of necleotide to MRNA

A

translation

24
Q

components of Translation

A

Ribosomes
Trna
Mrna

25
large complexes of protein and ribosimal Rna
Ribosomes
26
Ribosomes have 2 subunits
Large subunit and small subunit
27
3 attachment sites of ribosomes
A Site P site E site
28
what occupies P siye
peptidyl trna
29
this binds an incoming aminoacyl RNA and spcifies next amino acid to be added
A site
30
E site
exit site. occupied by empty TRNA
31
Translations | Factors
proteins synthesized by other genes
32
E. Elongation factprs
allows movement of the translation machinery
33
Aid in codon recognition
E. initiation factors
34
E. Release factors
recognizes stop codon and polypeptide from the machinery and aid in assembly of the complex
35
process of producing a functioning polypeptide
Translation
36
Quinolones
an antibiotic that targets dna replication
37
what is the function of quinolones
blocks topoismerase II and IV
38
MEtronidazle
inhibitor.. attaches itself to the DNA that cause it damage
39
Rifampicin
ttargets enzyme for RNA transcriptiion in the bacteria