DNA Flashcards
DNA
Deoxyribonucleic acid
4 base pairs
Adenine
Thymine (Uracil)
Cytosine
Guanine
Adenine and Thymine (Uracil) are purines (double ring)
Cytosine and Guanine are pyrimidines (single ring)
Nucleic Acid
Nucleic acids are long chains (polymers) made up of many nucleotide monomers joined together by phosphodiester bonds.
Each nucleotide is made up of:
-Pentose sugar (either deoxyribose or ribose),
-Nitrogenous base (guanine, cytosine, adenine, thymine or uracil)
-Phosphate
Formation of polynucleotide
Between phosphate group of one nucleotide and sugar of another
Condensation reaction
Phosphodiester bond
Bonds between bases
Hydrogen bonds
G and C (triple hydrogen bond)
A + T (double hydrogen bond)
DNA Replication
Enzyme helicase breaks hydrogen bond between bases and separates the 2 polynucleotide strands with unzipping action
Free nucleotides line up with complementary base pairs and hydrogen bonds form with bases
Polymerase attaches nucleotides together (joining the phosphate group)
DNA in Eukaryotic vs Prokaryotic
Eukaryotic
-Nucleus
-Associated with histones
-Linear
-Longer
-No plasmids
Prokaryotes
-Not in nucleus
-Not associated with histones
-Circular
-Shorter
-Plasmids
DNA in chloroplasts and mictochondria similar to DNA in prokaryotes
Advantages of DNA being double stranded
Replication
Stability
Protect the bases
Chromosome
Made up of chromatin which is a tightly coiled combination of proteins and DNA
Genome
Complete set of genetic material in an organism
Proteome
Complete set of proteins that can be expressed by a genome at a certain time