DNA Flashcards

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1
Q

DNA

A

Deoxyribonucleic acid

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2
Q

4 base pairs

A

Adenine
Thymine (Uracil)

Cytosine
Guanine

Adenine and Thymine (Uracil) are purines (double ring)
Cytosine and Guanine are pyrimidines (single ring)

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3
Q

Nucleic Acid

A

Nucleic acids are long chains (polymers) made up of many nucleotide monomers joined together by phosphodiester bonds.

Each nucleotide is made up of:
-Pentose sugar (either deoxyribose or ribose),
-Nitrogenous base (guanine, cytosine, adenine, thymine or uracil)
-Phosphate

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4
Q

Formation of polynucleotide

A

Between phosphate group of one nucleotide and sugar of another

Condensation reaction

Phosphodiester bond

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5
Q

Bonds between bases

A

Hydrogen bonds

G and C (triple hydrogen bond)
A + T (double hydrogen bond)

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6
Q

DNA Replication

A

Enzyme helicase breaks hydrogen bond between bases and separates the 2 polynucleotide strands with unzipping action

Free nucleotides line up with complementary base pairs and hydrogen bonds form with bases

Polymerase attaches nucleotides together (joining the phosphate group)

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7
Q

DNA in Eukaryotic vs Prokaryotic

A

Eukaryotic
-Nucleus
-Associated with histones
-Linear
-Longer
-No plasmids

Prokaryotes
-Not in nucleus
-Not associated with histones
-Circular
-Shorter
-Plasmids

DNA in chloroplasts and mictochondria similar to DNA in prokaryotes

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8
Q

Advantages of DNA being double stranded

A

Replication
Stability
Protect the bases

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9
Q

Chromosome

A

Made up of chromatin which is a tightly coiled combination of proteins and DNA

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10
Q

Genome

A

Complete set of genetic material in an organism

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11
Q

Proteome

A

Complete set of proteins that can be expressed by a genome at a certain time

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