Dna. Flashcards
DNA Is
A stable information carrying molecule, that stores genetic code (Deoxyribonucleic acid)
Genetic code is
The information which instructs a cell which proteins to make
DNA is a polymer of
Monomer subunits called nucleotides
Nucleotides contain what atoms
C, H, O, N, and P
What are the three parts of nucleotides
-Phosphate group
-Deoxyribose sugar
-Nitrogenous base
(All joined by covalent bonds)
How is single-strand DNA formed
When nucleotides join together in a chain by covalent bonds which form between the phosphate group of one nucleotide and the deoxyribose of the other, forming a sugar-phosphate backbone.
How is a double-stranded DNA formed
when 2 single strands of DNA twist around each other to form a double helix which is held together by hydrogen bonds between the nitrogenous bases of nucleotides on different strands
Are the 2 strands parallel
They are anti parallel (run parallel to each other but in opposite directions)
What are the 4 nitrogenous bases in DNA
Adenine, Cytosine, Guanine, and Thymine
What structural groups do these nitrogenous bases belong to
Adenine and guanine are purines (2 rings containing nitrogen), Cytosine and thymine are pyrimidines (1 ring containing nitrogen)
What are the complimentary pairs and why
A and T (both form 2 hydrogen bonds)
C and G ( both form 5 hydrogen bonds)
Describe how the structure of DNA is related to its functions
-The sugar phosphate backbone provides strength and stability to protect the bases and hydrogen bonds.
-It’s a long molecule and its coiled and compact so it can store lots of information.
-It’s base sequence allows information to be stored.
-Double stranded so replication can occur.
-complimentary base pairing so accurate identical copies can be made.
-Hydrogen bonds are weak so can easily be broken for replication.
-Many hydrogen bonds are strong.
Compare the roles of RNA and DNA
DNA stores genetic material whereas RNA transfers genetic information from DNA in the nucleus to ribosomes in the cytoplasm, where proteins are made.
Compare the structures of DNA and RNA
-RNA is single stranded, DNA is double stranded
-RNA is shorter and less stable
-RNA is made up of nucleotides containing the nitrogenous bases adenine, cytosine, guanine, and uracil.
-Pentase sugar in RNA is ribose.
Compare the roles, structure, and diagram of mRNA and tRNA
Role:
-mRNA carries specific nucleotide bases to ribosomes.
-tRNA carries specific amino acids to ribosomes.
Structure:
-mRNA is single stranded and its codons (every 3 bases) bind to complimentary tRNA on anticodons.
-tRNA is single stranded (clover leaf), These strands pair on the same strand, its anticodon binds to a complimentary codon on mRNA.
Describe the steps of DNA replication
DNA helical enzyme unwinds the DNA double helix by breaking the H bonds which hold the strands together.
Bases on each DNA are exposed.
Each strand acts as a template. Free nucleotides attach to the exposed bass on each strand: A to T and C to G (complimentary base pairing)
DNA polymerase enzyme joins ides with phosphodiester bonds to form new strands.
H bonds form between the bases of the old and new strands.
Two new daughter DNA helices are made, each one an exact replica of the original (parent) helix.
Explain why DNA replication is semi-conservative
One old parent helix is replaced by two identical daughter helixes.
Each daughter helix contains one old DNA strand and one new DNA strands meaning both parent single strands have been conserved.
What is a gene
A sequence of DNA nucleotide bases that code for a polypeptide