DNA

Question 1

What is PCR polymerase chain reaction?

Answer 1

is the method used to make millions of copies of DNA from a small sample.

Question 2

How is sometimes PCR called?

Answer 2

molecular photocopiying

Question 3

How is DNA unzziped

Answer 3

Is unzziped by heating the sample in a mixture of DNA NUCLEOTIDES

Question 4

What is taq polymerase

Answer 4

amplifies the specific DNA sequence

Question 5

What is a primer

Answer 5

a short piece if RNA that starts the chain reaction

Question 6

DNA equation

Answer 6

original DNA strand —-heat——DNA u zips———polymerase enzymes

Question 7

What is a simple definition of biutechnology

Answer 7

a process to make millions of copies of DNA

Question 8

In which example is the fingerprint identical to the twins?

Answer 8

DNA fingerprints

Question 9

What does pcr produce

Answer 9

copies of dna

Question 10

What are the ingredients for the PCR process

Answer 10

DNA, primer, nucleotides, taq polymerase

Question 11

Where did biotechnology find the special DNA polymerase enzyme used in the pcr process

Answer 11

thermal pools

Question 12

What is a pcr primer

Answer 12

a little tab that matches DNA, and codes a specific region of DNA

Question 13

What are restriction enzymes

Answer 13

restriction enzymes are a natural defense that bacteria use to destroy viruses that invaded them

Question 14

What doesrestriction enzymes do

Answer 14

cut longpieces of DNA into smaller pieces

Question 15

When is DNA cut

Answer 15

wherever there is a recognition site which is determined by our genetics.

Question 16

What difuses faster

Answer 16

small things

Question 17

Whatdifuses slower

Answer 17

long pieces

Question 18

What are relps

Answer 18

is a type of polymorphism that results from variation in the DNA sequence recognized by restriction enzymes

Question 19

When are relps separated from each other

Answer 19

during a process called gel electrophoresis

Question 20

What are gel electrophoresis

Answer 20

is a precise method for separating pieces of DNA called rflps or strs that have been cut by restriction enzymes. It produces a visual gel record

Question 21

Whatchargeis DNA

Answer 21

DNA has a slightly negative charge

Question 22

How are DNA pieces separated

Answer 22

by gel electrophoresis

Question 23

What does pcr do

Answer 23

amplifies the original I’m a sample

Question 24

What are recognition sites

Answer 24

nucleotide sequences

Question 25

What are rflps and strs

Answer 25

restriction fragment length polymorphism

Question 26

Why not decode the entire DNA of the person or organism being investigated

Answer 26

because it will take a long time

Question 27

What I a the purpose of the gel

Answer 27

to separate the DNA

Question 28

What is the purpose of the buffer

Answer 28

To break the DNA

Question 29

Where do you put the DNA samples

Answer 29

in the gel holes

Question 30

When the electrical charge is applied to the gel, what happens

Answer 30

bubbles are created and the color is separated

Question 31

What are called the targeted sequence

Answer 31

genetic markers

Question 32

What are probes

Answer 32

special molecules designed to combined only with a particular sequence of nucleotides in the DNA sample

Question 33

How do you make gel

Answer 33

preparing a agarose gel sheet in a casting tray, cover the gel with a buffer solution, place the sample to be compared into small wells at one end if the gel sheet, apply and electrical charge to each end of the gel, wait to the DNA fingerprints to develop

Question 34

What is agar made of

Answer 34

seaweed

Question 35

making gel

Answer 35

measure 40 mo of buffer and pour it into the flask, predetermined the weight of a piece of withing paper of 0.35 agar, add it to the flask swirl it and heat it up in the microwave for 15 seconds. When the flak has cooled you pour it into the gel tray with the comb in place. Once the gel has hardened remove the comb and the ends of the gel tray and insert it into the main chamber. Once the gel is in the chamber you will inject samples using a micropippete and wait for the samples to separate into bands.