Diversity of Protists Flashcards
Eukaryotes:
protists, plants, animals, fungi
Have membrane-bound organelles
• Have a nucleus
• Have a cytoskeleton for support (Allows for asymmetrical cell forms)
4 supergroups within Eukarya (2 unresolved protistan group)
most are single celled organisms
Protists
are the most abundant eukaryotic lineages and are unicellular
Some can exist in colonies or be multicellular
Diverse reproductive cycles
Asexual, sexually, alternating between 2N and N
Describe the nutritional modes in protists
Diverse nutritional modes:
Photoautotrophs, containing chloroplasts
Heterotrophs
Mixotrophs, both photosynthetic and heterotrophic( Have arisen independently in protist lineages )
Explain how the endomembrane system and plastids evolved in the evolution of eukaryotes.
i
Compare primary and secondary endosymbiosis.
a. Identify the number of separate genomes in organisms with plastids.
i
Describe the basic characteristics of each of the four Supergroups.
a. Classify protists into one of the Supergroups or smaller clades (see list of names at the end of this document) based on present characteristics.
i
Supergroup
i
Heterotroph
i
Autotroph
i
Mixotroph
both photosynthetic and heterotrophic
Endomembrane system
i
Primary endosymbiosis
engulfing of a photosynthetic cyanobacterium by a heterotrophic eukaryote
First transfer of photosynthesis from a prokaryote to a eukaryote
Secondary endosymbiosis
heterotrophic eukaryote ate a photosynthetic eukaryote
Usually a red or green algae
Spread photosynthesis to 3 out of 4 supergroups
Photosynthetic eukaryote becomes the new plastid
Plastid
is a membrane-bound organelle found in the cells of plants, algae, and some other eukaryotic organisms. They are considered to be intracelluar endosymbiotic Cyanobacteria
Nucleomorph
i
Phytoplankton
diatom
subgroup Stramenopiles
supergroup SAR
Zooplankton
Unicellular organisms that move via cilia
cilliates
subrgoup alveolates
supergroup SAR
Accessory pigment
i
Macronucleus
transcriptional active (produces RNA) in cilliates
Micronucleus
exchange of genetic material during conjugation
Pseudopodia
false feet
in rhizorans
Alga
i
Amoeba
i
Plasmodium (syncytium)
parasite that causes malaria
subgroup; apicomplexan
Colonial
i
Endosymbiosis
is a symbiotic relationship where one thing lives inside another
Examples of Endosymbiosis
Mitochondria used to be a heterotrophic, O2 using prokaryote
All derived from a common ancestor
Chloroplasts used to be a photosynthetic prokaryote
• Arose multiple times
Name the supergroups of eukaryotes?
4 supergroups Excavata SAR Archaeplastida Unikonts
Supergroup Excavata
Excavates have an “excavated” feeding groove, based on cytoskeletal morphology
What are the monophyletic clades of Excavata?
3 monophyletic clades:
• Diplomonads
• Parabasalids
• Euglenozoans
Describe the Diplomonads
Mitochondria: mitosomes (lack functional ETC)
Metabolism: anaerobic pathways
Nutritional Mode: parasites (ex; giardia intestinalis beaver fever)
NUclei; 2 equal sized
Flagella: Multiple
Other features:
Describe the Parabasalids
Mitochondria: reduced called hydrogenosomes
Metabolism: anaerobic releasing H as by product
Nutritional Mode: parasite (ex; Trichomonas vaginalis STI)
NUclei
Flagella: 2
Other features: undulating membranes
Describe the Euglenozoans
Mitochondria: Single, large (Kinetoplastids)
Metabolism: *
Nutritional Mode: Predatory heterotrophs, autotrophs, mixotrophs, parasites
Nuclei: *
Flagella: Crystalline rod inside one of their flagella
Other features: Light detectors (Euglenids)
Supergroup SAR
Very large group defined by DNA similarities (biggest and most diverse)
(Excavata was based on morphology )
What are the 3 monophyletic clades of SAR?
Stramenopiles
Alveolates
Rhizarians
What is included in the clade Stramenopiles?
Diatoms
Brown algae
Oomcyetes
What is included in the clade Alveolates ?
Dinoflagellates
Apicomplexans
Ciliates
What is included in the clade Rhizarians ?
Forams
Radiolarians
Cercozoans
What are the diagnostic features of subgroup Stramenopiles?
2 flagella
1 with hairlike projections 1 smooth
What are the diagnostic features of subgroup Alveolates?
Membrane bound sac (Alveoli) under the plasma membrane
What are the diagnostic features of subgroup Rhizarians?
Amoebas and flagellated protists with pseudopodia
Diatoms
subgroup Stramenopiles
Unicellular algae with a silica covering made of two pieces
~10,000 species
Phytoplankton
Main cause of shellfish poisoning due to the toxins they produce
Can result in huge “blooms”
Brown Algae
subgroup Stramenopiles
Multicellular, mostly marine “seaweeds’
Alternation of generations life cycle (plant life cycle)
Brown due to carotenoids (accessory pigment) in their plastids
Have specialized structures such as
Holdfasts – anchorage
Stipe – “stem”
Blades – “leaves”
Above arose independently from similar features in plants -> analogous structures
Golden algae and oomycetes (used to be called water moulds(fungal but new molecular evidence found they look more like protists) are also included in this group
Dinoflagellates
Subgroup Alveolates
Cells are reinforced by cellulose plates (groove in the middle)
Contains 2 flagella within grooves (they spin when they move)
Photoautotrophic (through secondary endosymbiosis),
mixotrophic and heterotrophic aquatic organisms
Causes “red tide”, blooms in the ocean
Can produce toxins
Apicomplexans
Subgroup Alveolates
Unicellular parasites of animals
Contains sporozoites, specialized cells that invade host cells
Ex. Plasmodium, parasite that causes malaria
Have complex life cycles with asexual and sexual stages and which require 1-2 hosts
Ciliates
Subgroup Alveolates
Unicellular organisms (zooplankton) that move via cilia
Most are predators that feed on bacteria and algae
Have 2 nuclei:
Micronucleus -> exchange of genetic material during conjugation
Macronucleus -> transcriptional active (produces RNA)
Reproduce via binary fission
Radiolarians
Subgroup Rhizarians
Have delicate, symmetrical internal silica skeletons
Pseuodpodia(thin) radiate from central body
Mostly marine
Foraminiferans(Forams)
Subgroup Rhizarians
Name means “little hole” for their porous shells (tests)
Made of calcium carbonate
Pseudopodia extend through the pores for moving, feeding, and building the shell
Aquatic, both marine and freshwater
90% of all forams are known from fossils
Cercozoans
Subgroup Rhizarians
Amoeboid and flagellated protists
Common in marine, freshwater and soil ecosystems
Heterotrophs (most) and parasites (many) and predators
Describe the Supergroup Archaeplastida
Composed of red algae, green algae and land plants
Monophyletic clade
common ancestor is an ancient protist that engulfed a cyanobacterium
Red Algae
~600 species, red due to accessory pigment phycoerythrin (allows it to absorbs blue or green light)
Absorbs blue and green light
Most abundant large algae in tropical marine habitats
Multicellular, still considered “seaweeds”
Commonly eaten as a food source (Nori in sushi)
Green Algae
~7000+ species
Single-celled and multicellular
Green due to chlorophyll in their chloroplasts
Paraphyletic group:
Chlorophytes -> mostly freshwater and terrestrial species
• Can be unicellular, multicellular and/or colonial
Charophytes -> closely related to land plants (next topic)
Describe the Supergroup Unikonts ? what are the2 major clades
Includes animals, fungi, and some protists
Two major clades:
Amoebozoans
Opisthokonts
Each clade is supported by molecular systematics and analysis of myosin proteins
However, we don’t know the root of the Eukaryotic tree at the Unikonts
• We don’t know which group of Eukaryotes came first
What do the Amoebozoans include?
Slime Moulds
Tubulinids
Entameobas
what do the Opisthokonts include?
Nucleariids
Fungi
Choanoflagellates
Animals
Amoebozoans
Includes amoebas with lobe or tube-shaped pseudopods
What 2 forms do slime moulds occur in?
Plasmodial slime moulds
Cellular slime moulds (single cell)
Slime Moulds
( Mycetozoans,formerly Myxomycota)
Produce fruiting bodies that aid in spore dispersal
But no longer considered fungi -> convergent evolution
Plasmodial slime moulds
Form a mass called plasmodium (syn. syncytium), a single- celled cytoplasm containing many nuclei
Produced via mitosis that does not end in cytokinesis
Extends pseudopodia through substrate to feed (phagocytosis)
When environment dries, stops expanding and produces sporangia
Cellular slime moulds
Feeding stage consists of solitary cells but join together when food is depleted, forming aggregated amoebas that produce stalked sporangia for spore dispersal
Tubulinids
Unicellular protists found in soil and aquatic environments
Most are heterotrophs, eating bacteria and other protists
Ex Amoeba proteus
Free-living
Entamoebas
Parasites, infecting vertebrates
Ex. Entamoeba histolytica causes amoebic dysentery, which is spread by contaminated water
What 2 groups remain uncertain and why?
There are two major groups of protists that remain uncertain
So hard to isolate and culture, grouping them based on morphology and/or DNA is near impossible
• Haptophytes
• Cryptomonads
What is the strongest evidence of the EST?
Residual nuclei (nucleomorphs) are strongest evidence of EST Rhizaria and cryptonomads