Disk-diffusion Assay

Question 1

What is penicillins target?

Answer 1

peptidoglycan

Question 2

What is tetracyclines target?

Answer 2

ribosome & inhibiting protein translation

Question 3

What is erythromycins target?

Answer 3

ribosome & inhibiting protein translation

Question 4

What is a disk-diffusion assay used for?

Answer 4

to determine effectiveness of chemical agents against a particular microbe

Question 5

What does the zone of inhibition measure?

Answer 5

how effective that antimicrobial is against the particular species being tested

Question 6

What are broad spectrum antimicrobials effective against?

Answer 6

a wide range of species including both gram negative & gram positive

Question 7

What are narrow spectrum antimicrobials effective against?

Answer 7

specific species

Question 8

Is penicillin narrow or broad spectrum?

Answer 8

narrow spectrum

Question 9

What is penicillin effective towards?

Answer 9

gram positive species

Question 10

When does antagonism occur?

Answer 10

when the effect of two drugs together is less than the effect of either alone

Question 11

When does synergism occur?

Answer 11

when the effect of two drugs together is greater than the effect of either alone