discrepancies

Question 1

weak/ missing antibody

Answer 1

-most common
from: patient age, immune status, current/past diseases

-recent plasma exchange: AB plasma doesn’t have anti-b or anti-a

Question 2

resolution for weak antibody/ missing

Answer 2

add 1-2 drops of plasma and incubate at RT longer

Question 3

unexpected antibody

Answer 3

causes: cold reactive antibodies, rouleaux, anti-a1, passively acquired anti-a or anti-b from platelets

Question 4

report type as A2

Answer 4

if patient serum shows no reactivity with dolichos lectin nad no reactivity with A2 cells

Question 5

if screening cell is positive at IS phase its

Answer 5

cold alloantibody

Question 6

if all cells have reactivity including screening cells and auto control

Answer 6

cold autoantibody

Question 7

cold alloantibody resolution

Answer 7

run full panel since screening cell negative and antigen type units for negative antigen you found against plasma of patient

Question 8

cold autoantibody resolution

Answer 8

report cold autoantibody or some places runs warm settled cell OR autoabsorb

Question 9

cold autoantibody settled cell reading

Answer 9

set up plasma with A1 cells, B cells and SC1, incubate 37 degrees for 1 hour and read agg.

Question 10

weak/missing antigen

Answer 10

cause: recent transfusion with type O cells that reduced reactivity/mixed field, leukemia, HPC transplants from group O donor

Question 11

weak/missing antigen solution

Answer 11

increase RT incubation time, incubate 4 C include group O cell and autocontrol

Question 12

extra antigens

Answer 12

HPC transplant with incompatible donor (ABO is 2nd to HLA in marrow transplant)– continues to make donor type

Question 13

extra antigen resolutions

Answer 13

HPC transplant– confirm patient history and given history type

Question 14

acquired B phenotype

Answer 14

acidifying human anit-b eliminates reactivity with acquired b-cells but keeps reactivity with true B cells (give type A cells)

Question 15

bombay will show reactivity

Answer 15

anti-H, anti- A, anti-B cause has nothing

Question 16

bombay and group o cells are

Answer 16

incompatible (O has H)

Question 17

why is Rh testing invalid or history does not match?

Answer 17

1) patient is weak d +
2) patient has rouleaux- auto control positive
3) patient’s DAT +
4) mixed field on cord blood (contamination from mother)

Question 18

weak d

Answer 18

<3 + investigated; only care now if donor unit or cord blood has weak testing

Question 19

weak d test resolution

Answer 19

will positive once you take it all the way to AHG phase

Question 20

rouleaux resolution and reasoning

Answer 20

when Rh control is positive — wash patient’s cells and repeat test

Question 21

patient DAT resolution

Answer 21

tubes will have identical agg. ; suspect poor washing and perform DAT again

Question 22

if donor unit has positive DAT

Answer 22

quarantine and report to blood center

Question 23

if cord blood positive DAT

Answer 23

check mother’s type and screen – send to reference lab