Direct Microscopic Exam Flashcards

1
Q

3 uses of direct microscopic examination

A

Detection of fungi
Confirmation of fungi
Identification of fungi (based on morphological appearance)

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2
Q

Method that softens tissues and dissolves fat droplets and other debris

A

Wet Mount Preparation

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3
Q

A mounting fluid that softens keratin and dissolves fat droplets

A

KOH

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4
Q

10% KOH is used for ___ tissues

A

Soft

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5
Q

20% KOH is used for ___ tissues

A

Hard

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6
Q

Component of KOH that prevents evaporation

A

Glycerine

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7
Q

(True or False)

10% KOH is composed of 10 mg KOH pellets

A

False (10 g)

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8
Q

Best method for Pneumocystis

A

Immunofluorescence microscopy

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9
Q

SDA w/ Chloramphenicol (SDC) inhibits the growth of ____ & ____

A

E.coli & A.niger

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10
Q

Selective medium used for the isolation of fungi from specimen heavily contaminated w/ bacteria and saprophytic fungi

A

BHI w/ Chloramphenicol & Gentamicin (BHICGA)

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11
Q

Medium used in suppression of saprophytes and cultivation of dermatophytes

A

Mycobiotic agar w/ Actidione

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12
Q

Most common medium used to isolate fungi

A

SDA

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13
Q

3 antibiotics added to SDA

A

Chloramphenicol
Gentamicin
Tetracyline

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14
Q

It is a source of amino acids

A

Peptones

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15
Q

Source of energy and carbon

A

Dextrose

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16
Q

(True or False)

Gentamicin is against gram-positive bacteria

A

False (gram-negative)

17
Q

Pasty opaque, slightly domed, smooth, creamy-white colonies

A

C. albicans

18
Q
Yellow-green powdery (front)
Pale yellowish (reverse)
A

A. flavus

19
Q

Gives a “salt and pepper” appearance

A

A. niger

20
Q
Bluish-green powdery (front)
Pale yellow (reverse)
A

A. fumigatus

21
Q

Wrinkled, white-cream, yellowish colonies

A

Trichosporon mucoides

22
Q

Flat, white-cream, w/ aerial mycelium

A

Geotrichum candidum