Direct Microscopic Exam Flashcards
3 uses of direct microscopic examination
Detection of fungi
Confirmation of fungi
Identification of fungi (based on morphological appearance)
Method that softens tissues and dissolves fat droplets and other debris
Wet Mount Preparation
A mounting fluid that softens keratin and dissolves fat droplets
KOH
10% KOH is used for ___ tissues
Soft
20% KOH is used for ___ tissues
Hard
Component of KOH that prevents evaporation
Glycerine
(True or False)
10% KOH is composed of 10 mg KOH pellets
False (10 g)
Best method for Pneumocystis
Immunofluorescence microscopy
SDA w/ Chloramphenicol (SDC) inhibits the growth of ____ & ____
E.coli & A.niger
Selective medium used for the isolation of fungi from specimen heavily contaminated w/ bacteria and saprophytic fungi
BHI w/ Chloramphenicol & Gentamicin (BHICGA)
Medium used in suppression of saprophytes and cultivation of dermatophytes
Mycobiotic agar w/ Actidione
Most common medium used to isolate fungi
SDA
3 antibiotics added to SDA
Chloramphenicol
Gentamicin
Tetracyline
It is a source of amino acids
Peptones
Source of energy and carbon
Dextrose