Diagnostic Sampling (Small Animals) Flashcards
Successful venipuncture requires ______ and _______
Proper restraint + Visualization/immobilization of the vessel
What materials are needed for venipuncture blood sample collection?
- Needle + syringe
- Butterfly catheter with or without syringe or vacutainer
- Vacutainer collection system
What makes up a vacutainer collection system?
- Double-pointed needle
- Plastic holder
- Blood collection tube(s) with or without anticoagulant
Vacutainer Collection System
VP collection site, method of collection, and needle gauge is chosen based on:
1.
2.
3.
4.
- Vessel size
- Amount of blood (sample) required
- Intended use of the sample
- VT preference
Needle Sizes: Small Gauge
25 to 28 gauge
Needle Sizes: Medium/Average Gauge
22 gauge
Needle Sizes: Large Gauge
18 and 20 gauge
When should you use 25-28 gauge needles?
- Smaller, fragile vessels
- Multiple/frequent VPs
When should you use 22 gauge needles?
- VP on cats
- VP on small dogs
When should you use 18 and 20 gauge needles?
- VP on large breed dogs
- VP on most farm animals
the method of blood sample collection in which the colored-top tubes are filled in a certain order to allow for minimal contamination or “carry over” between tubes
Order of Draw
Blood collected from VP must be _______ and _______.
placed into a specific colored-top tube + filled in order of draw
PROCEDURE - Putting Collected Blood in Appropriate Tubes
- Removing the needle from the collection apparatus + the stopper from the tube
- Blood flows down the side of the blood collection tube to the “fill line” as blood is gently expelled from the collection device
- Tube’s contents are adequately mixed by gently rocking the tube back and forth a predetermined number of times
Why is it important to NOT vigorously mix or forcefully eject collected blood samples?
Can cause hemolysis of sample
the destruction of red blood cells
Hemolysis
Always label blood collection tubes with:
1.
2.
3.
- Patient’s first + last names
- Date of collection
- Owner’s first + last names
Give 3 examples of blood sample coagulation profiles
- Activated Clotting Time (ACT)
- Prothrombin Time (PT)
- Activated Partial Thromboplastin Time (APTT)
Why is it important to break the seal of the syringe plunger before venipuncture?
May cause:
1. Vein to collapse
2. RBC hemolysis
Needle is always inserted with bevel facing _____ in all VP procedures
up
PROCEDURE - Venous Blood Collection (Needle + Syringe Method)
- Attach a 20-25 gauge needle to a 1-6 mL syringe
- Occlude the vein with a tourniquet or digital pressure
- Wipe the skin and hair on top of the vein with an alcohol-soaked cotton ball to help identify the vein
- Break the plunger seal by pulling it half way out of the syringe
- Insert the needle with the bevel facing up through the skin and into the vein at a 25º angle
- Slowly retract the syringe plunger + collect a blood sample
- Release the pressure on the vein + release the syringe plunger when a sufficient volume of blood has been collected
- Remove the needle from the vein
- Apply digital pressure to the venipuncture site as soon as the needle is removed > until hemostasis occurs
PROCEDURE - Cephalic Venipuncture
- Place patient in sternal or lateral recumbency
- Grasp the leg at the metacarpus > extends the foreleg
- Occlude the vessel
- Pop the syringe plunger seal
- VT grasps the right foreleg of the patient with nondominant hand at the carpus > wipes the VP site with 70% isopropyl alcohol
- VT digitally palpates the vessel > rest thumb of nondominant hand directly against + parallel to the vessel
- Insert the needle along the vein’s long axis on the other side from your thumb
- “Flash” of blood should be noted within the needle hub > stop insertion > gently pull syringe plunger to obtain predetermined amount of blood
- Remove needle while gently aspirating
- Apply pressure to insertion areas for 30 sec > evaluate site for bleeding and/or hematoma formation for several minutes
If bleeding continues:
– Apply pressure bandage – Report clotting delay to attending vet
- Place collected blood in appropriate blood collection tubes
Cephalic Vein
Why should the cephalic vein be the last choice for diagnostic sample collection?
Vessels need to be maintained for IVs if needed
Give 3 scenarios in which jugular VP would be used
- Chemistry profiles
- Hyperthyroid testing
- Cushing test
Give 4 scenarios in which jugular VP would NOT be used
Patients with:
1. Head trauma
2. Possible heat stroke
3. Clotting abnormalities
4. Snake bites
PROCEDURE - Jugular Venipuncture
- Place patient in sternal, lateral, or seated recumbency
- Grasp underneath either side of patient’s jaws > gently extend the head toward ceiling
- Apply digital pressure at thoracic inlet > occludes vessel
- Pop the syringe plunger seal
- Wipe the VP site with 70% isopropyl alcohol
- VT digitally palpates the vessel > rest thumb of nondominant hand directly against + parallel to the vessel
- Insert the needle along the vein’s long axis on the other side from your thumb
- “Flash” of blood should be noted within the needle hub > stop insertion > gently pull syringe plunger to obtain predetermined amount of blood
- Remove needle while gently aspirating
- Apply pressure to insertion areas for 30 sec
- Place collected blood in appropriate blood collection tubes
If patient is in sternal recumbency > jugular VP is done in the _______ direction.
cephalic
If patient is in lateral recumbency > jugular VP is done in ________ direction.
caudal
PROCEDURE - Lateral Saphenous Venipuncture DOGS
- Place patient in lateral recumbency
- Grasp the upper leg at the stifle > extends the hindlimb
- Apply circumferential pressure to the stifle of the top hindleg > occludes the vessel
- Pop the syringe plunger seal
- VT grasps the top hindleg of the patient using nondominant hand at at the metatarsus > wipes the VP site with 70% isopropyl alcohol
- VT digitally palpates the vessel > rest thumb of nondominant hand directly against + parallel to the vessel
- Insert the needle along the vein’s long axis on the other side from your thumb
- “Flash” of blood should be noted within the needle hub > stop insertion > gently pull syringe plunger to obtain predetermined amount of blood
- Remove needle while gently aspirating
- Apply pressure to insertion areas for 30 sec > evaluate site for bleeding and/or hematoma formation for several minutes
- If bleeding continues:
– Apply pressure bandage
– Report clotting delay to attending vet
- Place collected blood in appropriate blood collection tubes
PROCEDURE - Lateral Saphenous Venipuncture CATS
- Place patient in lateral recumbency
- Grasp the upper leg with thumb just cranial to the patella + index finger is in the patella fold on the caudal aspect of the rear leg > extends the hindlimb
- Apply upward circumferential pressure behind + caudal to the patella > occludes the vessel
- Clipping the hair in the stifle area (just cranial to the tarsal bone) may aid in vein visualization - Pop the syringe plunger seal
- VT grasps the top hindleg of the patient using nondominant hand at at the metatarsus > wipes the VP site with 70% isopropyl alcohol
- VT digitally palpates the vessel > rest thumb of nondominant hand directly against + parallel to the vessel
- Insert the needle along the vein’s long axis on the other side from your thumb
- “Flash” of blood should be noted within the needle hub > stop insertion > gently pull syringe plunger to obtain predetermined amount of blood
- Remove needle while gently aspirating
- Apply pressure to insertion areas for 30 sec > evaluate site for bleeding and/or hematoma formation for several minutes
- If bleeding continues: – Apply pressure bandage – Report clotting delay to attending vet - Place collected blood in appropriate blood collection tubes
Femoral vein is also known as the _____ vein.
Medial Saphenous Vein
PROCEDURE - Medial Saphenous Venipuncture for DOGS
- Place patient in lateral recumbency
- Grasp the upper leg at the stifle > extends the leg
- Apply circumferential pressure to the stifle of the bottom hindleg > occludes the vessel
- Pop the syringe plunger seal
- VT grasps the bottom hindleg of the patient using nondominant hand at at the metatarsus > wipes the VP site with 70% isopropyl alcohol
- VT digitally palpates the vessel > rest thumb of nondominant hand directly against + parallel to the vessel
- Insert the needle along the vein’s long axis on the other side from your thumb
- “Flash” of blood should be noted within the needle hub > stop insertion > gently pull syringe plunger to obtain predetermined amount of blood
- Remove needle while gently aspirating
- Apply pressure to insertion areas for 30 sec > evaluate site for bleeding and/or hematoma formation for several minutes
-If bleeding continues: – Apply pressure bandage
- Report clotting delay to attending vet
PROCEDURE - Medial Saphenous Venipuncture for CATS
- Place patient in lateral recumbency
- Grasp the top hind leg > abduct + flex the upper leg > exposes medial surface of bottom hind leg
- Apply pressure with the side of your abducting hand along the inguinal area of the bottom hindleg (“karate chop” fashion) > occludes the vessel
- Pop the syringe plunger seal
- VT grasps the bottom hindleg of the patient using nondominant hand at at the metatarsus > wipes the VP site with 70% isopropyl alcohol
- VT digitally palpates the vessel > rest thumb of nondominant hand directly against + parallel to the vessel
- Insert the needle along the vein’s long axis on the other side from your thumb
- “Flash” of blood should be noted within the needle hub > stop insertion > gently pull syringe plunger to obtain predetermined amount of blood
- Remove needle while gently aspirating
- Apply pressure to insertion areas for 30 sec > evaluate site for bleeding and/or hematoma formation for several minutes
- If bleeding continues: – Apply pressure bandage
- Report clotting delay to attending vet
Which species is VP of the marginal ear vein primarily used for? And what two things does it test for?
Rabbits - To test for:
1. Erythropoiesis
2. Blood glucose levels
PROCEDURE - Marginal Ear Venipuncture
- Apply warmth to ear vein with a heated cloth for approx. 30 sec > dilates vessel
- Heated cloth
- Light source
- VTs hands - Apply 70% isopropyl alcohol to VP site
- Slightly nick the skin + vein with a 25-gauge x 3/4” needle or lancet
- Once blood appears on skin surface > massage pinna to obtain sufficient blood sample
- Apply direct pressure to stop bleeding for approx. 15 sec
Venipuncture of the Marginal Ear Vein
ABG Sampling is short for _______
Arterial Blood Gas Sampling
ABG samples are used to assess:
1.
2.
3.
4.
- Pulmonary function
- Gas exchange within the lungs
- Measurement of blood gases (O2 and CO2)
- Blood pH
Give 3 examples of what ABG samples can help diagnose
- Kidney failure
- Heart failure
- Diabetic ketoacidosis
List the 4 most common sites used to obtain an ABG sample
- Dorsal pedal artery
- Dorsal metatarsal artery
- Femoral artery
- Sublingual artery (unconcious/anesthetized patients)
What two kinds of needles + syringes can be used to collect an ABG sample?
- 22 or 25 gauge needle + 1 or 3 mL syringe + lithium or sodium heparin
- ABG self-filling syringe coated with lithium heparin
PROCEDURE - Arterial Blood Collection
- Coat dead space of a syringe (with attached needle) with heparin > expel excess heparin from the syringe
- Use appropriate restraint
- VT digitally palpates the target vessel with nondominant hand
- Site is clipped and/or surgically prepped
- Using palpating fingers to guide > insert needle with heparinized syringe bevel side up + parallel to the artery + at 45º angle
- Flash of blood should be noted within the needle hub
- Fill syringe
- If ABG self-filling syringe > syringe will automatically fill to a predetermined volume set prior to venipuncture
- If lithium heparin-coated syringe + needle > VT gently pulls syringe plunger to obtain 1-1.5 mL sample - Remove needle gently + apply pressure to insertion areas for 1 min > monitor for hematoma formation
- Expel all air from the collection syringe + place rubber stopper on end of the needle
- Sample placed in ice water bath during transport to lab for immediate analysis OR sample can stay in ice water bath for several hours before analysis
What are 2 important things to avoid doing when collecting an ABG sample? Why?
- Introducing air
- Applying excessive negative pressure
Both affect PaO2 measurements
The medial saphenous vein can be easily confused with the _______.
femoral artery
_____ is the preferred artery for arterial catheter placement.
Dorsal Metatarsal Artery
What 2 scenarios would you use arterial catheter placement?
- Collection of multiple blood samples
- Continuous measurement of arterial BP
PROCEDURE - Arterial Catheter Placement
- Place patient in lateral recumbency + extend hock
- Site is clipped + aseptically prepped
- Flush a 20-22 gauge over-the-needle (OTN) catheter with heparinized normal saline
- Palpate the artery
- Using needle’s beveled edge > make a relief hole in dermis for insertion
- Position the catheter bevel-up + subcutaneously above the artery
- Palpate needle tip + artery simultaneously with fingers of opposite hand
- Insert the catheter steeply until it penetrates the upper artery wall > then move it flat against the skin surface + parallel to the artery’s long axis
- Flash of blood should be seen within the needle hub > then carefully advance the catheter into the artery to its full length
- Replace needle with a T-connector + stopcock
- Wrapping adhesive tape around the hub to secure catheter > Cover the insertion site with a 2”x2” piece of gauze > Apply roll gauze followed by tape
- Flush catheter every 2 hours with heparinized saline OR attach to a continuous flush system
- Check toes for warmth every 2-4 hours
-If toes are cool > remove catheter - Perform catheter care every 48 hours
_____ mL needed for most quantitative urinalyses
7-10 mL
_____ mL needed for strip-based urinalyses
< 7 mL
What temperature should samples be for urinalysis if they have been refrigerated?
Room temperature
List 4 methods of urine sample collection
- Voided collection
- Manual bladder expression
- Cystocentesis
- Urinary catheterization
method of urine sample collection in which the sample is gathered as or after the patient excretes the urine and is used for routine urinalysis
Voided Collection
Voided urine sample collections are NOT acceptable for ______.
Cultures
method of urine sample collection in which the sample is gathered by applying pressure directly to the bladder and is used in animals that have difficulty voiding due to neurological deficits
Manual Bladder Expression
Manual bladder expression sample collections are NOT acceptable for ______.
Cultures
Do NOT use manual bladder expression for ______. Why?
Urinary blockages > may result in urethral or vesicular rupture
PROCEDURE - Manual Bladder Expression
- Place patient in standing or lateral recumbency
- VT places hands on either side of the caudal abdomen
- Isolate the bladder between the palmar surfaces of the fingers
- Gently and continuously apply pressure to express the urine
- If method does not work with moderate compression > MUST use a different method
method of urine sample collection in which the sample is gathered by placing a needle through the abdominal wall to draw urine directly from the bladder and is used to obtain sterile urine samples for analysis or culture and sensitivity testing
Cystocentesis
Give 4 scenarios in which cystocentesis would be used
- Urinalysis
- Culture and sensitivity testing
- Aid in localization of hematuria, pyuria, bacteriuria
- Relieve distended bladder if catheter cannot be placed
blood cells in the urine
Hematuria
pus in the urine
Pyuria
Give 4 scenarios in which cystocentesis is NOT used
Patients with:
1. Recent abdominal surgery or trauma
2. Suspected bleeding disorders
3. Pyometra
4. Suspected caudal abdominal or bladder tumor
uterine infection
pyometra
Why is it important to remove most but not ALL urine during a cystocentesis?
Removing all volume risks contact between needle + bladder wall > damage to bladder
During a cystocentesis, insert the needle _______ of the bladder instead of ______.
During a cystocentesis, insert the needle [cranial to the trigone region] of the bladder instead of [the apex].
Why should you NEVER redirect the needle once in the abdominal cavity during a cystocentesis?
May result in accidental viscera laceration
Give 3 possible complications of a cystocentesis
- Bladder laceration
- Bowel laceration
- Peritonitis (as a result of prior)
Give 2 contraindications for cystocentesis
- Inadequate urine volume in bladder
- Patient resists restraint + abdominal palpation
What size neddle + syringe are needed for a cystocentesis?
- 22-gauge x 1-1.5 in needle
- 12 mL or larger syringe
Preferred restraint(s) for ventrolateral cystocentesis
- Standing recumbency
- Lateral recumbency
Preferred restraint(s) for ventral cystocentesis
- Dorsal recumbency
PROCEDURE - Ventrolateral Cystocentesis
- Place patient in standing or lateral recumbency
- Palpate abdomen to determine size + location of bladder
- Hold the syringe with the needle in one hand + stabilize the bladder from below with the free hand
- Press bladder dorsally + caudally to immobilize it against the pelvis - Wipe insertion site with alcohol
- Insert needle caudomedially into abdominal cavity + bladder > 45º angle to bladder wall + toward cranial trigonal region
- Aspirate urine in the syringe until desired sample volume is obtained
- Stop aspiration or release negative pressure on the plunger of syringe BEFORE withdrawing the needle
PROCEDURE - Ventral Cystocentesis
- Place patient in dorsal recumbency
- May take 2 assistants for large breeds or deep-chested dogs - Palpate abdomen to determine size + location of bladder
- Hold the syringe with the needle in one hand + stabilize the bladder from below with the free hand
- Press bladder dorsally + caudally to immobilize it against the pelvis - Wipe insertion site with alcohol
- Insert needle on midline into abdominal cavity > 45º angle to bladder wall + directed caudally
- Aspirate urine in the syringe until desired sample volume is obtained
- Stop aspiration or release negative pressure on the plunger of syringe BEFORE withdrawing the needle
method of urine sample collection in which the sample is gathered by placing a tube directly into the urethra
Urinary Catheterization
List 4 possible complications of urinary catheterization
- Urethral inflammation
- Bacterial UTI
- Urethral and bladder irritation
- Trauma
What 2 scenarios would indwelling urinary catheters be used?
Patients that have:
1. Recently had urethral calculi removed
2. Neurologic impairment/traumatic conditions interfering with normal urination
having normal blood pressure
Normotensive
having a normal volume of blood in the body
Normovolemic
A normotensive, normovolemic patient with intact renal function has a urinary output of _____ per _____ per hour.
1-2 mL of urine per kg of body weight