Diagnosing viral infection Flashcards
1
Q
2 main approaches
A
Viral detection - electron microscopy, PCR
Serology testing - ELISA, immunofluorescence
2
Q
What does viral detection and serology testing test for
A
- Viral detection - do you have virus or not - viral presence
- Serology - do you have the antibodies or complement that shows you have virus present - immune response
3
Q
PCR features
A
- False positive
- Need to suspect what virus patients have for PCR
- Cheap
- Sensitive
- Quick
4
Q
Diagnostic swabs
A
Green viral swab + black charcoal swab
Green - virus
Black - bacteria
5
Q
Shingles
A
- Painful rash confined to single dermatome
- A single line of rash across eg T4 - think Shingles
- Causes by VZV
6
Q
EBV
A
Causes infections mononucleosis
DD is S. pyogenes
Both present with tonsilitis - yellow whitish purulent lining of tongue
7
Q
How do you differentiate between EBV and s pyogenes
A
Charcoal swab
8
Q
You dont use green swab for EBV what do you use
A
- Use blood test to find atypical lymphocytes
- Serology of this is EBV
- Check for Igg or IgM
- IgM - acute infection
- IgG - past infection - have secondary memory
9
Q
If you see atypical lymphocytes what should you think?
A
EBV
10
Q
Infectious mononucleosis symptoms
A
Cough
Fever
Splenomegaly
11
Q
Tests for IM
A
Blood film
FBC
Eliza testing
12
Q
Strep pyogenes other tests
A
- Anti streptolysin titre - shows how much AB produced against toxin
- Produces streptolysin - body produces anti - strepsolysin - more AB you have more severe infection is
13
Q
Tx for IM
A
- Fluids
- Analgesia
- Avoid contact sports for 6 weeks
14
Q
Bronchoalveolar lavage fluid
A
- Fluid collected from lungs
- Sent for analysis in PCR and microscopy and cultures
15
Q
CMV
A
- AIDS-defining illness
- Causes CMV colitis
- See owl eye inclusion bodies in colon
