Diabetes Flashcards
insulin synthesis
- cleave the C-peptide off proinsulin (presence of C-peptide indicates endogenous synthesis of insulin)
- stimulated by presence of glucose & amino acids (leucine, arginine) & acetylcholine -> acts on phospholipase C-IP3 pathway -> increase intracellular Ca2+
insulin receptor
- insulin binds to the alpha receptor -> autophosphorylation and activation of tyrosine kinase in beta subunit
- phosphorylates IRS-1 protein
- activates PI3-kinase: PIP2 -> PIP3
- PIP3 binds to Akt -> recruitment of GLUT4
insulin fn
maintains normal blood glucose levels - facilitate glucose uptake in cells - regulate carbs. lipid, protein metab increase glycolysis, decrease lipolysis, increase protein synthesis in muscle - promote cell division and growth
insulin clearance
kidney: GFR and proximal tubular reabsorption
types of DM
- type 1 DM: absolute insulin def - immune mediated beta-cell destruction so cannot produce insulin
- type 2 DM: insulin resistance
- gestational DM - glucose intolerance during pregnancy
type 1 DM treatment
daily insulin injections
type 2 DM treatment
lifestyle modification (diet, exercise)
insulin therapy indications
type 1 DM
type 2 DM: only in severe hyperglycemia / OHAs not useful
insulin therapy drugs
mimicks normal pancreatic insulin secretion
- basal insulin: suppresses hepatic production of glucose
- prandial: provides insulin to remove excess serum glucose after eating
5 groups of insulin
- rapid-acting insulins
- short-acting insulins
- intermediate-acting
- long-acting
- premixed
rapid acting insulin drugs
insulin analogues (has the letter s in it)
- lispro
- aspart
- glulisine
rapid acting insulin analogues MOA
change to charge and conformation of the insulin molecule
reduce repulsion -> more rapid absorption = shorter duration of action
injected just before meals
short acting insulin MOA
similar to regular human insulin - crystalline zinc insulin
injected 20-30min before meals
clear appearance
greater hypoglycemia risk than rapid acting insulin
intermediate acting insulin drug
neutral protamine hagedorn (NPH)
NPH MOA
protamine + human insulin
precipitated crystals of NPH insulin releases slowly -> slower onset
- high risk of hypoglycemia cause of the variability from the long onset of action
so must eat after medication
cloudy appearance
long acting insulin drugs (2)
- glargine
- detemir - binds insulin to albumin: prolongs insulin action
acts for 18-24hrs
clear appearance
insulin degludec
ultra long acting (U-LA) insulin - slow release of insulin
42hrs duration of action
because of the long duration, adjustments not easy -> esp for those that need dosage adjustment cause of renal/hepatic failure
insulins that should NOT be mixed
- glargine (LA) -> incompatible pH
- detemir (LA)
- glulisine (RA) - exc that it can be mixed w/ NPH
insulins that can be mixed
- most insulins can be mixed w/ NPH
- aspart (RA) + degludec (U-LA)
factors affecting pk of insulin
- faster absorption through abdomen injection
- inject into muscles
- exercise increase rate of absorption
- heat
DM caused by drugs
steroids: hyperglycemia
ADR caused by insulin therapy
- risk of hypoglycemia if too much insulin is given
dizziness, tremor, weakness, hunger
esp in renal impaired pts/ elderly - lipodystrophy (abnormal fat distribution)
lipoatrophy: loss of fat at the site of the injection
lipohypertrophy: accumulation of fat due to repeated injections at the same - lipogenic effect of insulin
methods to monitor blood glucose
- casual plasma glucose (>11.1mmol/l)
- fasting plasma glucose (>7mmol/l)
- 2 hour post challenge plasma glucose
- HbA1c - glycated haemoglobin (reflects 2-3mths blood glucose lvls)
drugs to help maintain blood glucose levels in type 2 DM (insulin resistance) - MOA
- increase sensitivity of tissues to insulin
- reduce glucose release by liver
- slow down carbs digestion in intestines
- inhibit glucagon (glucagon inhibits insulin secretion)
- stimulate insulin release
- incretins ??
- block glucose reabsorption at kidneys
groups of drugs to increase sensitivity of tissues to insulin (2)
- biguanide
- thiazolidinediones