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DEHYDRATION Flashcards

1
Q

typical tissue starts at what concentration

A

70% ethanol

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2
Q

May be used in delicate specimens; for eyes and embryo

A

ETHANOL

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2
Q

delicate tissue (embryo) begins at what concentration

A

30% ethanol

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2
Q

Excellent substitute for ethanol

A

ISOPROPANOL

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2
Q

Extracts/removes methylene blue and other thiazine dyes from sections

A

ETHANOL

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2
Q

Best dehydrating agent

A

ETHANOL

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2
Q

temperature in butanol

A

117.7 C

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2
Q

Recommended and most common for routine
dehydration

A

ETHANOL

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3
Q

Sufficiently water-free to use in place of absolute ethanol

A

ISOPROPANOL

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3
Q

Recommended for tissues which do not require rapid processing

A

BUTANOL

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3
Q

May react with unreduced osmium tetroxide (OsO4)

A

ETHANOL

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3
Q

Blood, touch preparations, tissue film preparations, and smear preparations

A

METHANOL

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3
Q

Mixes with water, ethanol, xylene, and paraffin in all

A

TERTIARY BUTANOL

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3
Q

Long periods of infiltration necessary

A

BUTANOL

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3
Q

plant and animal micro-techniques

A

BUTANOL

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3
Q

Does not harm tissue over long time periods

A

DIOXANE

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3
Q

May be used in staining series as a dehydrating agent

A

TERTIARY BUTANOL

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3
Q

other name of DIOXANE

A

DIETHYLENE DIOXIDE

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3
Q

Reagent tends to solidify at room temperature or below 25C

A

TERTIARY BUTANOL

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3
Q

other name for pentanol

A

(AMYL ALCOHOL)

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3
Q

Miscible with 90% alcohol, toluene and xylene

A

PENTANOL (AMYL ALCOHOL)

3
Q

Microwave processing

A

ISOPROPANOL

3
Q

Cannot be used in the celloidin technic since
nitrocellulose is insoluble in it

A

ISOPROPANOL

3
Q

Does not extract methylene blue and other dyes from stained sections

3
Not reactive with OsO4 remaining in specimen
ACETONE
3
Dissolves paraffin wax
PENTANOL (AMYL ALCOHOL)
3
Cannot be used for preparing staining solutions, since dyes are not soluble in it
ISOPROPANOL
4
Best processing requires a graded series of a mixture of acetone and xylene before one can go into paraffin
ACETONE
4
Fixative and dehydrating agent
ACETONE
4
Miscible with water, alcohol, xylene, and paraffin
DIOXANE
4
dehydrating agent that is Highly toxic
DIOXANE
4
Miscible with most embedding resin
ACETONE
4
dehydrating agent that is cheap and rapid
ACETONE
4
Ribbons poorly
DIETHYLENE DIOXIDE
4
Faster dehydrant than ethanol
DIOXANE
4
Distorts tissue-containing cavities (e.g lungs)
DIOXANE
4
dehydrating agent that has peroxides
DIOXANE
4
Toxic: reproductive, fetal, urinary, blood system
CELLOSOLVE
4
tissue is wrapped in a gauze bag and suspended in a bottle containing dioxane and a little anhydrous calcium oxide
Weiseberger's method
4
Ethylene glycol ether combust at
110-120F (43-48C)
4
Rapidly absorbs water from the air
CELLOSOLVE
5
Rapid dehydrating agent
CELLOSOLVE
5
Requires clearing agent
CELLOSOLVE
5
May be used in routine paraffin technique
TRIETHYL PHOSPHATE
6
Displaces water readily with slight distortion
TRIETHYL PHOSPHATE
7
Soluble in alcohols, benzene, toluene, xylene, ether, chloroform
TRIETHYL PHOSPHATE
8
May be used as a dehydrating solution in the staining sequence
TRIETHYL PHOSPHATE
9
Miscible in all proportions with water, ether, chloroform, acetone, and the hydrocarbons xylene, toluene, and benzene
TETRAHYDROFURAN
10
>6months exposure produces conjunctival irritation
TETRAHYDROFURAN
11
ADDITIVES TO DEHYDRATING AGENTS
GLYCEROL, PHENOL
12
2 softening agent
GLYCEROL, PHENOL

HISTOCYT (4 decks)

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