DEHYDRATION Flashcards
typical tissue starts at what concentration
70% ethanol
May be used in delicate specimens; for eyes and embryo
ETHANOL
delicate tissue (embryo) begins at what concentration
30% ethanol
Excellent substitute for ethanol
ISOPROPANOL
Extracts/removes methylene blue and other thiazine dyes from sections
ETHANOL
Best dehydrating agent
ETHANOL
temperature in butanol
117.7 C
Recommended and most common for routine
dehydration
ETHANOL
Sufficiently water-free to use in place of absolute ethanol
ISOPROPANOL
Recommended for tissues which do not require rapid processing
BUTANOL
May react with unreduced osmium tetroxide (OsO4)
ETHANOL
Blood, touch preparations, tissue film preparations, and smear preparations
METHANOL
Mixes with water, ethanol, xylene, and paraffin in all
TERTIARY BUTANOL
Long periods of infiltration necessary
BUTANOL
plant and animal micro-techniques
BUTANOL
Does not harm tissue over long time periods
DIOXANE
May be used in staining series as a dehydrating agent
TERTIARY BUTANOL
other name of DIOXANE
DIETHYLENE DIOXIDE
Reagent tends to solidify at room temperature or below 25C
TERTIARY BUTANOL
other name for pentanol
(AMYL ALCOHOL)