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HISTOCYT > DEHYDRATION > Flashcards

DEHYDRATION Flashcards

1
Q

typical tissue starts at what concentration

A

70% ethanol

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2
Q

May be used in delicate specimens; for eyes and embryo

A

ETHANOL

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2
Q

delicate tissue (embryo) begins at what concentration

A

30% ethanol

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2
Q

Excellent substitute for ethanol

A

ISOPROPANOL

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2
Q

Extracts/removes methylene blue and other thiazine dyes from sections

A

ETHANOL

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2
Q

Best dehydrating agent

A

ETHANOL

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2
Q

temperature in butanol

A

117.7 C

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2
Q

Recommended and most common for routine
dehydration

A

ETHANOL

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3
Q

Sufficiently water-free to use in place of absolute ethanol

A

ISOPROPANOL

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3
Q

Recommended for tissues which do not require rapid processing

A

BUTANOL

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3
Q

May react with unreduced osmium tetroxide (OsO4)

A

ETHANOL

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3
Q

Blood, touch preparations, tissue film preparations, and smear preparations

A

METHANOL

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3
Q

Mixes with water, ethanol, xylene, and paraffin in all

A

TERTIARY BUTANOL

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3
Q

Long periods of infiltration necessary

A

BUTANOL

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3
Q

plant and animal micro-techniques

A

BUTANOL

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3
Q

Does not harm tissue over long time periods

A

DIOXANE

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3
Q

May be used in staining series as a dehydrating agent

A

TERTIARY BUTANOL

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3
Q

other name of DIOXANE

A

DIETHYLENE DIOXIDE

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3
Q

Reagent tends to solidify at room temperature or below 25C

A

TERTIARY BUTANOL

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3
Q

other name for pentanol

A

(AMYL ALCOHOL)

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3
Q

Miscible with 90% alcohol, toluene and xylene

A

PENTANOL (AMYL ALCOHOL)

3
Q

Microwave processing

A

ISOPROPANOL

3
Q

Cannot be used in the celloidin technic since
nitrocellulose is insoluble in it

A

ISOPROPANOL

3
Q

Does not extract methylene blue and other dyes from stained sections

3
Q

Not reactive with OsO4 remaining in specimen

3
Q

Dissolves paraffin wax

A

PENTANOL (AMYL ALCOHOL)

3
Q

Cannot be used for preparing staining solutions, since
dyes are not soluble in it

A

ISOPROPANOL

4
Q

Best processing requires a graded series of a mixture
of acetone and xylene before one can go into paraffin

4
Q

Fixative and dehydrating agent

4
Q

Miscible with water, alcohol, xylene, and paraffin

4
Q

dehydrating agent that is Highly toxic

4
Q

Miscible with most embedding resin

4
Q

dehydrating agent that is cheap and rapid

4
Q

Ribbons poorly

A

DIETHYLENE DIOXIDE

4
Q

Faster dehydrant than ethanol

4
Q

Distorts tissue-containing cavities (e.g lungs)

4
Q

dehydrating agent that has peroxides

4
Q

Toxic: reproductive, fetal, urinary, blood system

A

CELLOSOLVE

4
Q

tissue is wrapped in a gauze bag and suspended in a bottle containing dioxane and a little anhydrous calcium oxide

A

Weiseberger’s method

4
Q

Ethylene glycol ether combust at

A

110-120F (43-48C)

4
Q

Rapidly absorbs water from the air

A

CELLOSOLVE

5
Q

Rapid dehydrating agent

A

CELLOSOLVE

5
Q

Requires clearing agent

A

CELLOSOLVE

5
Q

May be used in routine paraffin technique

A

TRIETHYL PHOSPHATE

6
Q

Displaces water readily with slight distortion

A

TRIETHYL PHOSPHATE

7
Q

Soluble in alcohols, benzene, toluene, xylene, ether,
chloroform

A

TRIETHYL PHOSPHATE

8
Q

May be used as a dehydrating solution in the staining sequence

A

TRIETHYL PHOSPHATE

9
Q

Miscible in all proportions with water, ether,
chloroform, acetone, and the hydrocarbons xylene, toluene, and benzene

A

TETRAHYDROFURAN

10
Q

> 6months exposure produces conjunctival irritation

A

TETRAHYDROFURAN

11
Q

ADDITIVES TO DEHYDRATING AGENTS

A

GLYCEROL, PHENOL

12
Q

2 softening agent

A

GLYCEROL, PHENOL

HISTOCYT (4 decks)

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