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HISTOCYT > CLEARING > Flashcards

CLEARING Flashcards

1
Q

aka dealcoholization

A

CLEARING

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2
Q

Clearing agents are fully miscible to

A

ethanol and paraffin wax, and mounting media

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2
Q

Remove a substantial amount of fat from the tissue which otherwise presents a barrier to wax infiltration

A

CLEARING

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2
Q

done after dehydration or before infiltration; after staining or before mounting

A

CLEARING

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2
Q

Removal of dehydrant

A

CLEARING

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3
Q

gives structure back to the tissue

A

paraffin

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3
Q

how many changes of xylene in clearing

A

2 changes of xylene
- xylene (1st: 25mins), xylene (2nd: 45mins)

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3
Q

most important and critical part of tissue
processing because as much as possible it should preserve the tissue
in the living state.

A

Fixation

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3
Q

unique aliphatic substitute for xylene

A

Pro-par clearant

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3
Q

acts as an intermediate solvent/middle man so
paraffin could fully infiltrate the tissue

A

Clearing agent

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3
Q

specimen thickness in clearing

A

4mm

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3
Q

cause brittleness and more difficult to cut

A

Prolonged clearing:

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3
Q

fully miscible with both ethanol
and paraffin wax

A

Intermediate solvent

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3
Q

a good cleaning and clearing agent

A

xylene

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3
Q

specimen vol in clearing

A

20x the tissue volume/size

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4
Q

how many changes for Pro-par clearant

A

3 changes (45mins, 45mins, 45mins

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4
Q

Factors affecting Clearing

A

boiling point, viscosity and refractive index

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4
Q

It evaporates quickly in paraffin oven and can,
therefore, be readily replaced by wax during
impregnation and embedding

A

. XYLENE/XYLOL

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4
Q

Suitable for urgent biopsies which it clears within 15-30 minutes.

A

. XYLENE/XYLOL

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4
Q

It is the most rapid and common clearing agent

A

. XYLENE/XYLOL

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4
Q

substitute for xylene or benzene

A

TOLUENE

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4
Q

Toxic: CNS, Resp, Integumentary, Liver dse,
nephrotoxicity, conjunctivitis, teratogenic and fetotoxic

A

. XYLENE/XYLOL

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4
Q

better at preserving tissue structure

A

TOLUENE

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4
Q

Routine clearing agent

A

. XYLENE/XYLOL

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5
Q

Highly concentrated solutions will emit fumes that are toxic upon prolonged exposure

A

TOLUENE

6
Q

Slower than xylene and benzene

A

TOLUENE

6
Q

It volatilizes rapidly in paraffin oven and is therefore easily eliminated from the tissue

A

BENZENE

6
Q

It volatilizes rapidly in paraffin oven and is therefore easily eliminated from the tissue

A

BENZENE

6
Q

It is rapid acting, hence is recommended for urgent biopsies (15-60mins)

A

BENZENE

7
Q

it is recommended for tough/dense tissues leg skin, uterus, muscle, fibroid and decalcified tissues for nervous tissues, lymph nodes

A

CHLOROFORM

7
Q

Extremely carcinogenic to man causing APLASTIC ANEMIA

A

BENZENE

7
Q

It is suitable for large tissue specimen

A

CHLOROFORM

7
Q

Miscible with absolute alcohol

A

CHLOROFORM

7
Q

Tissues tend to float

A

CHLOROFORM

7
Q

Cause less brittleness than xylene

A

CHLOROFORM

7
Q

Toxic to the liver, should be used in well-ventilated room

A

CHLOROFORM

7
Q

clearing agent For delicate specimens

A

ANILINE OIL

7
Q

Its vapor may attack the rubber seal used in vacuum
impregnating bath

A

CHLOROFORM

7
Q

Embryos (bouin’s), insects,

A

ANILINE OIL

8
Q

it has a dangerous toxic gas

A

CHLOROFORM

8
Q

For central nervous system tissues and cytological studies, particularly of smooth muscles and skin

A

CEDARWOOD OIL

8
Q

clearing agent that has offensive odor

A

TETRAHYDROFURAN

8
Q

Extremely slow clearing agent (plus days)

A

CEDARWOOD OIL
2-3 days (paraffin)
5-6days (celloidin)

8
Q

clearing agent that Makes tissue brittle

A

CLOVE OIL

8
Q

clearing agent from plant extracts

A

TERPENES

8
Q

Similar to chloroform but cheaper

A

CARBON TETRACHLORIDE (CCl4) OIL

8
Q

using graupner’s , how many hours/ minutes using paraffin

A

3x in 90min

8
Q

clear both paraffin and celloidin section

A

CEDARWOOD OIL

8
Q

clearing agent that is non toxic

A

TETRAHYDROFURAN

8
Q

clearing agent where Celloidin is dissolved

A

CLOVE OIL

8
Q

Superior to dehydrating and clearing agents

A

TETRAHYDROFURAN

8
Q

clearing agent where aniline dyes are removed

A

CLOVE OIL

8
Q

using graupner’s , how many hours/ minutes using dioxane

A

3x in 4hrs

8
Q

Prevent hepatotoxin

A

CARBON TETRACHLORIDE (CCl4) OIL

9
Q

clearing agent for Double embedding

A

METHYL BENZOATE/SALICYLATE

9
Q

Also called as Esters

A

METHYL BENZOATE/SALICYLATE

10
Q

clearing agent that is Good for manual processing

A

METHYL BENZOATE/SALICYLATE

11
Q

clearing agent for Papanicolaou smears

A

CARBO-XYLENE

12
Q

Temp. of paraffin: in clearing

A

50-85C

12
Q

clearing agent for Thick mucinous

A

. CARBO-XYLENE

HISTOCYT (4 decks)

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