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HISTOCYT > DECAL > Flashcards

DECAL Flashcards

1
Q

aka demineralization

A

DECALCIFICATION

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2
Q

calcium or lime salts are removed from tissues
following fixation

A

DECALCIFICATION

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3
Q

Wash the decalcified
specimen for

A

24-48 hrs

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4
Q

Quantity of decal solution:

A

> 20 vol. of specimen

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5
Q

most common and fastest decal agent

A

5%-10% Nitric Acid

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5
Q

Rapid acting, good nuclear staining decal agent

A

Formol-Nitric Acid

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6
Q

Lessen yellow tissue discoloration by 5% sodium sulfate or 0.1% urea

A

Formol-Nitric Acid

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6
Q

Decalcifying and tissue softener but slow acting

A

Perenyi’s

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6
Q

silver impregnation of nerve fibers

A

De Castro’s Fluid

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6
Q

slower, greater tissue distortion decal agent

A

Hydrochloric Acid (HCl)

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6
Q

Most rapid, poor nuclear staining

A

Phloroglucin-Nitric Acid

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7
Q

Must be removed by 3 changes of 70-90% ethanol

A

Phloroglucin-Nitric Acid

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7
Q

duration for small specimens

A

1 to 3 weeks

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7
Q

calcium ions are attracted to a

A

negative electrode (cathode)

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7
Q

Simple, reliable and recommended for routine
purposes (test)

A

Chemical Test

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8
Q

best general decalcifying agent and fixative

A

5% Formic acid

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8
Q

Buffer solution for electrophoresis

A

8% HCL + 10% Formic acid

8
Q

Excellent nuclear and cytoplasmic staining

A

5% Formic acid

8
Q

Very weak, for minute bone pieces

A

Sulfurous Acid

9
Q

Slow; not recommended for urgent examinations.

A

Trichloroacetic Acid (TCA)

9
Q

duration for dense cortical bone

A

6 to 8 weeks:

9
Q

Highly corrosive, carcinogenic and an environmental hazard

A

Chromic Acid (Flemming S)

9
Q
A
9
Q

Removal of calcium and exchanged with ammonium ions from ammonium-sulfonated polystyrene

A

ION EXCHANGE RESIN

9
Q

aka muriatic acid

A

Hydrochloric Acid (HCl)

9
Q

ph level that allows binding and does not destroy tissue components

A

pH 7.0 to 7.4 (neutral):

9
Q

Fixative and decalcifier

A

Chromic Acid (Flemming S)

10
Q

If there are small amounts =

A

ppt will show after 10 Mins

10
Q

uses ammonium-sulfonated polystyrene +
formic acid

A

Resins

10
Q

Dissolution of calcium by an acid

A

ION EXCHANGE RESIN

10
Q

safe choice for decalcification

A

*formic acid

10
Q

may cause swelling or make tissues
soapy

A

molliflex

10
Q

Calcified specimen which are 2-3mm thick could be decalcified within

A

2 to 3hrs

10
Q

for urgent diagnosis

A

for urgent diagnosis

10
Q

Ideal and reliable, expensive, not applicable on
mercuric chloride fixed tissues (test)

A

Radiologic test

10
Q

Washing Out: to remove acids in Small specimen

A

30 mins

10
Q

neutralization agent for post decal

A

2% LiCo
5 to 10% NaHCO3

10
Q

If large amount of calcium is present

A

= immediate
precipitation

10
Q

Washing Out: to remove acids
large specimen

A

1-4hrs

11
Q

Complete decalcification

A

Clear fluid

HISTOCYT (4 decks)

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