DEFINITION 2 Flashcards
same direction
PARALLEL β-SHEETS
different or opposite direction
ANTIPARALLEL β-SHEETS
For determination of n-terminal amino acid using 2,4,dinitroflurobenzene
Sanger method
Other reagents used to label N-terminus:
a. Dansyl chloride
b. Dabsyl chloride
Reagent that cleaves at carboxyl end of methionine
Cyanogenbromide
Types of mutation:
Deletion mutation
Insertion mutation
Point mutation
replacement of 1
Point mutation
replacement but same amino acid
silent mutation
replacement of different amino acid
missense mutation
premature stopping of codon. Replacement of stop codon
nonsense mutation
- Are soluble in aqueous system
- Complex tertiary structure
- Folded into a spherical/ globular shape
- Some have quarternary structure
Globular proteins
Globular proteins examples:
HEMOGLOBIN
ALBUMIN
GLOBULIN
PROTAMINES
INSULIN
HISTONES
used in transporting oxygen
HEMOGLOBIN
soluble in water and salt solution
ALBUMIN
plays important role in liver function and fighting infection
GLOBULIN
used to reverse and neutralize anticoagulant effects of heparin
PROTAMINES
regulatory hormone to control glucose level in bodies
INSULIN
protein that provides structural support for chromosomes
HISTONES
- Mainly primary and secondary structures
- Little or no tertiary structure
- Long parallel polypeptide chains
- Cross linkages at intervals
- Long fibers and sheets are formed
- Water insoluble and physically tough
- Serve as structural or protective elements
Fibrous proteins
Fibrous proteins examples
KERATIN
ELASTIN
MYOSIN
COLLAGEN
SILK
found in hair and outer layer of skin
KERATIN
present in tendons, arteries or other elastic tissues/ ligaments
ELASTIN
found in muscle tissues
MYOSIN
major constituent of skin, tendons, bones, blood vessels and connective tissues
COLLAGEN
can get from spiderwebs
SILK