Deconstructing the Genome Flashcards
1
Q
Cloning DNA steps
A
1 - ligation of fragments to cloning vectors creates recombinant DNA molecules
2 - Host cells take up and amplify vector-insert recombinants
2
Q
cloning vectors
A
allow us to select amplify and maintain specific gene fragments in bacteria
3
Q
common vectors
A
plasmids
phages
bacterial and yeast artificial chromosomes
4
Q
plasmids contain
A
origin of replication
selectable marker
restriction sites
5
Q
libraries
A
just a collection of cloned fragments
