Deck 5 Flashcards

1
Q

Immunoflourescence detects

A

Ag

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2
Q

Wavelength of immunofluorescence

A

380-750 nm

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3
Q

Boya’s method for detection of

A

Mycobacterium

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4
Q

Rabies detected by

A

Immunofluorescence

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5
Q

Haemagglutination detects

A

Virus (Ab)

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6
Q

PCR procedure

A

Amplification of DNA

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7
Q

What is real-time PCR

A

Amplification of DNA in real time

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8
Q

Media used for culture

A

Natural and synthetic

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9
Q

Cytopathic effect

A

Any change in cell culture caused by virus

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10
Q

Blocking ELISA is used for detection of

A

Ab.

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11
Q

Annealing temperature of PCR

A

45-60*C

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12
Q

Skin contamination is cleaned by

A

0.5% chloramine B

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13
Q

4 stages of phagocytosis

A

Chemotaxis, adherence, ingestion, digestion

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14
Q

Excision of histological sample

A

1 cm maximum

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15
Q

Blastotransformation is

A

Blastotransformation is the process by which small lymphocytes transform into larger, active lymphoblasts, typically in response to stimulation by antigens or mitogens, as part of the immune response.

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16
Q

Cell culture division

A

Primary and established

17
Q

Enzyme most commonly used in ELISA

A

Horseradish peroxidase

18
Q

Conjugation involves

A

Ig, serum and fluorochrome

19
Q

PCR is

A

Amplification of DNA fragments

20
Q

PCR is used for virus detection of

A

Genome