Day 8 - 01/10/14

Question 0

MHCs have APCs,

What are APCs?

Answer 0

Antigen Presenting Cells.

Question 1

What does MHC stand for?

Answer 1

Major Histocompatibility Complex

Question 2

What does TCR stand for?

Answer 2

T-Cell antigen Receptors

Question 3

How does TCR communicate with MHC?

Answer 3

TCR detects PEPTIDES of FOREIGN PROTEINS bound to MHC on the cell surface of APCs.

Question 4

What 3 main qualities does MHC have?

Answer 4

Determines

• Susceptibility to disease.
• Development of Autoimmunity
• Pathology associated with transplantation.

Question 5

What does POLYGENIC mean in context of MHC.

Answer 5

There are lots of MHC Class 1+2 genes in each Individual.

Question 6

What does POLYMORPHIC mean in context of MHC.

Answer 6

Alleles of MHC Class 1+2 genes have evolved within populations.

Question 7

Give Examples of Class 1 molecules.

Answer 7

K
D
L

Question 8

Give examples of Class 2 molecules.

Answer 8

IA

IE

Question 9

What does Class 1 molecule look like?

Answer 9

ALPHA-CHAIN encoded within MHC locus with a BETA2-MICROGLOBULIN NOT encoded.

Question 10

What does Class 2 molecule look like?

Answer 10

ALPHA CHAINS & BETA CHAINS,

Both encoded at MHC locus.

Question 11

What common feature does CLASS 1&2 molecules have?

Answer 11

OUTER EXTRACELLULAR DOMAIN has a GROOVE.

Question 12

What function does the Groove have?

Answer 12

It’s where the FOREIGN PEPTIDES are trapped during SYNTHESIS/TRACKING of MHC molecules.

Question 13

What is the Groove made up of?

Answer 13

Floor - 8 Beta Sheets

Sides - 2 Alpha Helixes

Question 14

What function does the BETA SHEET FLOOR have?

Answer 14

Site of PEPTIDE BINDING.

Question 15

What function does the ALPHA-HELICAL SIDES have?

Answer 15

Recognise peptide and TCR contact regions.

Question 16

What do CLASS 1 molecules present?

Answer 16

ENDOGENEOUS peptides derived from INTRACELLULAR PROTEINS or PATHOGENS.

Question 17

What do CLASS 1 molecules present to?

Answer 17

CD8+ Cytotoxic T-Cells.

Question 18

What do CLASS 2 molecules present?

Answer 18

EXOGENEOUS peptides derived from EXTRACELLULAR PATHOGENS.

Question 19

What do CLASS 2 molecules present to?

Answer 19

CD4+ Helper T-Cells

Question 20

Define Genotype.

Answer 20

Total Genetic information in genome

Question 21

Define Haplotype.

Answer 21

Collection of Genes at MHC.

Question 22

Mammals are Diploid.

What does that mean for the genotype at MHC?

Answer 22

Genotype = Maternal Haplotype + Paternal Haplotype

Question 23

Why is it good to have diverse MHC among Individuals.

Answer 23

Survival of species.

Some will die, some won’t.