D1H1

Question 1

definition of hematopoiesis

Answer 1

the regulated process of creating new blood cells in the body

occurs mostly in bone marrow

all blood cells start off as HSCs, then differentiate

Question 2

bone marrow aspirate vs biopsy

Answer 2

biopsy lets you see architecture

Question 3

progenitor colony assay

Answer 3

by seeing what colonies are made from cells, you can see what they were progenitors for

Question 4

At what point in RBC maturation does EPO act?

Answer 4

CFU-E

Question 5

2 niches for HSC

Answer 5

1) endosteal niche: for storage, when HSC in quiescent state

2) vascular niche: for “active” HSC, that have been mobilized to proliferate and differentiate

Question 6

How are dormant HSC activated and dislodged from their niche?

Answer 6

G-CSF (granulocyte colony stimulating factor)

Osteoclast activation:

• bone gets degraded, release HSC from niche
• induced by inflammatory mediators

Question 7

What does G-CSF stimulate?

Answer 7

Neutrophils

• differentiation
• release from bone marrow
• phagocytic, killing activity

HSCs
-get mobilized

Question 8

Thrombopoietin is a primary regulator of _____

Answer 8

platelet production

Question 9

Erythropoietin (EPO) is key to ____ production

Answer 9

RBC production

Question 10

EPO for anemia b/c…

Answer 10

anemia = not enough RBCs

EPO needed for RBC production

Question 11

G-CSF for neutropenia b/c…

Answer 11

neutropenia = not enough neutrophils (type of WBC)

G-CSF stimulates production of neutrophils

Question 12

autologous vs. allogeneic bone marrow transplant

Answer 12

autologous: own stem cells
allogeneic: donor’s stem cells