D1.2 Protein Synthesis Flashcards
What are the 3 types of RNA
- mRNA - messenger
- rRNA - ribosomal
- tRNA - transfer
What is transcription?
Product of mRNA, first set of instructions to build any protein it might need
Stages of transcription
- Initiation stage
- Elongation stage
- Termination stage
What is the sense strand?
5’ to 3’ (coding strand)
What is the anti sense strand?
3’ to 5’ (template strand)
What is a triplet?
A group of 3 bases on DNA
What is a codon?
A group of 3 bases on mRNA
What is the start codon?
AUG
What is the anti codon?
UAC
What is gene mutation?
When a mistake is made synthesizing DNA or RNA
What is a point mutation?
Involves the deletion, addition or replacement of a single nucleotide
Give an example of point mutation and some symptoms
Sickle cell anemia- weakness, extreme pain, heart attack
How is transcription controlled?
Limiting amount of mRNA
What is a promoter region?
RNA polymerase cannot bind to any part of a gene and start transcribing, so only happens here
What is a transcription factor?
Bind to the promoter regions, which allows RNA polymerase to bind
Difference between coding and non coding sequences
Coding- contain instructions for making proteins
Non-coding- do not directly code for proteins
What is a 5’ cap?
A special modification added to the 5’ end of a newly transcribed RNA molecule during transcription
What is a poly-A-tail?
Protects mRNA molecule from degradation
Difference between exons and introns
Exons- protein coding regions
Introns- non protein coding regions
What is splicing?
Involves removing the introns and joining the exons
What does alternative splicing do?
Results in different versions of proteins which will function differently
What binds on the P site?
Initiator tRNA
What binds on the A site?
Incoming tRNA
What binds on the E site?
tRNA after transferring its amino acid to the growing chain and exits the ribosome
What is the function of proteasome in the recycling of amino acids?
- Allows cells to maintain a supply of amino acids
- maintain correct concentration + get rid of any non functional