D L2: Introduction to Chromatography

Question 1

when did chromatography start?

Answer 1

100 years ago

Question 2

what is chromatography ?

Answer 2

A technique which allows the separation of a mixture of analyte molecules through their interaction with a stationary phase and a mobile phase.

Question 3

what is the stationary phase?

Answer 3

The chromatographic phase (solid or liquid) that is held and through which the mobile phase moves

Question 4

what is mobile phase?

Answer 4

The chromatographic phase that moves through the stationary phase, either a liquid or gas

Question 5

what does eluent mean?

Answer 5

solvent entering a column or moving up a plate, becomes an Eluate when it leaves

Question 6

whats elution?

Answer 6

process of passing a liquid/gas through a column

Question 7

whats the analyse/solute?

Answer 7

the molecule undergoing separation Columns are either packed or open tubular

Question 8

what is paper chromatogprahy used for ?

Answer 8

used to separate inks or food dyes

Question 9

in paper chromatography what is the stationary phase and what is the mobile phase?

Answer 9

SP - paper

MP - water

Question 10

Components in a mixture will move through the stationary phase at ____ _____ under the influence of a mobile phase.

Answer 10

different

rates

Question 11

if a molecule acts with only the stationary phase, will there be any form of chromotogrpahy?

Answer 11

no there will not be because there needs to be two phases

Question 12

if the analyte only reacts with the stationary phase where will it be ?

Answer 12

at the top

Question 13

are both phases needed for chromtogrpahy?

Answer 13

yes

Question 14

what is partition ?

Answer 14

the rate of movement of the analyte through the column is dependant upon the relative solubility of the analyte in the stationary phase and the mobile phase.

Question 15

what is adsorption?

Answer 15

the relative polarities of the analyte and the stationary phase determines the rate at which the analyte moves.

Question 16

what are the 5 types of chromotgraphy?

Answer 16

Adsorption chromatography
Partition chromatography
Ion-exchange chromatography
Molecular exclusion chromatography
Affinity chromatography

Question 17

what happens in normal phase chromatography?

Answer 17

it is when the stationary phase is more polar than the mobile phase

Question 18

what is reverse phase chromatography?

Answer 18

The stationary phase is less polar than the mobile phase

Question 19

what is Eluotropic strength ?

Answer 19

measure of a solvents ability to remove an analyte from the stationary phase

Question 20

what does the strength of a solvent depend on?

Answer 20

polarity of both of the stationary and mobile phases

Question 21

In Normal Phase chromatography ____ polar solvents have a higher eluotropic strength

Answer 21

more

Question 22

In Reverse Phase chromatography ____ polar solvents have a higher eluotropic strength

Answer 22

less

Question 23

When describing a solvent as ‘weak’ or ‘strong’ it is imperative that you state the _____ of the___ ____

Answer 23

nature

stationary phase

Question 24

what is solid phase extraction used for?

Answer 24

isolate analytes from a sample
concentrate analytes from a sample
pre clean dirty samples (matrix)

Question 25

wat is the SPE procedure?

Answer 25

prepare sample 
condition 
equilibrate 
load sample 
wash impurities
elute analyte